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BCS1L Protein (AA 2-419) (rho-1D4 tag)

Crystallography grade BCS1L Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3097757
  • Target See all BCS1L Proteins
    BCS1L (BCS1-Like (BCS1L))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-419
    Origin
    • 5
    • 2
    Human
    Source
    • 2
    • 2
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This BCS1L protein is labelled with rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    PLSDFILALK DNPYFGAGFG LVGVGTALAL ARKGVQLGLV AFRRHYMITL EVPARDRSYA WLLSWLTRHS TRTQHLSVET SYLQHESGRI STKFEFVPSP GNHFIWYRGK WIRVERSREM QMIDLQTGTP WESVTFTALG TDRKVFFNIL EEARELALQQ EEGKTVMYTA VGSEWRPFGY PRRRRPLNSV VLQQGLADRI VRDVQEFIDN PKWYTDRGIP YRRGYLLYGP PGCGKSSFIT ALAGELEHSI CLLSLTDSSL SDDRLNHLLS VAPQQSLVLL EDVDAAFLSR DLAVENPVKY QGLGRLTFSG LLNALDGVAS TEARIVFMTT NHVDRLDPAL IRPGRVDLKE YVGYCSHWQL TQMFQRFYPG QAPSLAENFA EHVLRATNQI SPAQVQGYFM LYKNDPVGAI HNAESLRR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human BCS1L Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product BCS1L Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    BCS1L (BCS1-Like (BCS1L))
    Alternative Name
    BCS1L (BCS1L Products)
    Synonyms
    BCS Protein, BCS1 Protein, BJS Protein, FLNMS Protein, GRACILE Protein, Hs.6719 Protein, MC3DN1 Protein, PTD Protein, h-BCS Protein, DDBDRAFT_0188274 Protein, DDBDRAFT_0266725 Protein, DDB_0188274 Protein, DDB_0266725 Protein, DDBDRAFT_0184131 Protein, DDBDRAFT_0266726 Protein, DDB_0184131 Protein, DDB_0266726 Protein, 9130022O19Rik Protein, zgc:56205 Protein, BCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone Protein, mitochondrial chaperone BCS1 Protein, mitochondrial chaperone bcs1 Protein, mitochondrial ATPase Protein, BCS1-like (yeast) Protein, BCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone L homeolog Protein, BC1 (ubiquinol-cytochrome c reductase) synthesis-like Protein, BCS1L Protein, AFUA_3G13000 Protein, CC1G_00820 Protein, CC1G_01181 Protein, CC1G_01608 Protein, CC1G_11035 Protein, CC1G_11036 Protein, Bm1_30135 Protein, bcs1lA Protein, bcs1lB Protein, Bcs1l Protein, bcs1l.L Protein, bcs1l Protein
    Background
    Chaperone necessary for the assembly of mitochondrial respiratory chain complex III. Plays an important role in the maintenance of mitochondrial tubular networks, respiratory chain assembly and formation of the LETM1 complex. {ECO:0000269|PubMed:18628306}.
    Molecular Weight
    48.6 kDa Including tag.
    UniProt
    Q9Y276
    Pathways
    SARS-CoV-2 Protein Interactome
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