PERP Protein (AA 1-193) (rho-1D4 tag)
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- Target See all PERP Proteins
- PERP (TP53 Apoptosis Effector (PERP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-193
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This PERP protein is labelled with rho-1D4 tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MIRCGLACER CRWILPLLLL SAIAFDIIAL AGRGWLQSSD HGQTSSLWWK CSQEGGGSGS YEEGCQSLME YAWGRAAAAM LFCGFIILVI CFILSFFALC GPQMLVFLRV IGGLLALAAV FQIISLVIYP VKYTQTFTLH ANPAVTYIYN WAYGFGWAAT IILIGCAFFF CCLPNYEDDL LGNAKPRYFY TSA
Sequence without tag. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PERP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
- Tag Location: C-terminal Rho1D4-tag
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- The protein is purified from the cleared cell lysate using Rho1D4 capture materials.
- Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- In solution (50 mM Hepes, pH 7.4; 100 mM NaCl; 0,1 % OGNG; 0,01% CHS)
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- PERP (TP53 Apoptosis Effector (PERP))
- Alternative Name
- PERP (PERP Products)
- Synonyms
- KCP1 Protein, KRTCAP1 Protein, PIGPC1 Protein, RP3-496H19.1 Protein, THW Protein, dJ496H19.1 Protein, 1110017A08Rik Protein, kcp1 Protein, krtcap1 Protein, pigpc1 Protein, thw Protein, fk24g11 Protein, wu:fk24g11 Protein, PERP Protein, PERP, TP53 apoptosis effector Protein, PERP, TP53 apoptosis effector S homeolog Protein, PERP Protein, Perp Protein, perp.S Protein, perp Protein
- Background
- Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway (By similarity). {ECO:0000250}.
- Molecular Weight
- 22.6 kDa Including tag.
- UniProt
- Q96FX8
- Pathways
- Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity
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