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PERP Protein (AA 1-193) (rho-1D4 tag)

Crystallography grade PERP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3105096
  • Target See all PERP Proteins
    PERP (TP53 Apoptosis Effector (PERP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-193
    Origin
    • 2
    • 1
    Human
    Source
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This PERP protein is labelled with rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MIRCGLACER CRWILPLLLL SAIAFDIIAL AGRGWLQSSD HGQTSSLWWK CSQEGGGSGS YEEGCQSLME YAWGRAAAAM LFCGFIILVI CFILSFFALC GPQMLVFLRV IGGLLALAAV FQIISLVIYP VKYTQTFTLH ANPAVTYIYN WAYGFGWAAT IILIGCAFFF CCLPNYEDDL LGNAKPRYFY TSA
    Sequence without tag.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PERP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).
    • Tag Location: C-terminal Rho1D4-tag

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. The protein is purified from the cleared cell lysate using Rho1D4 capture materials.
    2. Eluate fractions are analyzed by SDS-PAGE.
    3. Protein containing fractions are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In solution (50 mM Hepes, pH 7.4; 100 mM NaCl; 0,1 % OGNG; 0,01% CHS)
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PERP (TP53 Apoptosis Effector (PERP))
    Alternative Name
    PERP (PERP Products)
    Synonyms
    KCP1 Protein, KRTCAP1 Protein, PIGPC1 Protein, RP3-496H19.1 Protein, THW Protein, dJ496H19.1 Protein, 1110017A08Rik Protein, kcp1 Protein, krtcap1 Protein, pigpc1 Protein, thw Protein, fk24g11 Protein, wu:fk24g11 Protein, PERP Protein, PERP, TP53 apoptosis effector Protein, PERP, TP53 apoptosis effector S homeolog Protein, PERP Protein, Perp Protein, perp.S Protein, perp Protein
    Background
    Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway (By similarity). {ECO:0000250}.
    Molecular Weight
    22.6 kDa Including tag.
    UniProt
    Q96FX8
    Pathways
    Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity
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