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Solute Carrier Family 25 (Mitochondrial Carrier, Peroxisomal Membrane Protein, 34kDa), Member 17 (SLC25A17) (AA 1-307) protein (rho-1D4 tag) Protein

Crystallography grade SLC25A17 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3110190
$5,919.98
Plus shipping costs $45.00 and $24.00 dry ice
0.25 mg ABIN3110190
0.25 mg ABIN3110190
local_shipping Shipping to: United States
Delivery in 50 to 55 Business Days
  • Target
    Solute Carrier Family 25 (Mitochondrial Carrier, Peroxisomal Membrane Protein, 34kDa), Member 17 (SLC25A17)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-307
    Origin
    • 2
    • 1
    Human
    Source
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    rho-1D4 tag
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MASVLSYESL VHAVAGAVGS VTAMTVFFPL DTARLRLQVD EKRKSKTTHM VLLEIIKEEG LLAPYRGWFP VISSLCCSNF VYFYTFNSLK ALWVKGQHST TGKDLVVGFV AGVVNVLLTT PLWVVNTRLK LQGAKFRNED IVPTNYKGII DAFHQIIRDE GISALWNGTF PSLLLVFNPA IQFMFYEGLK RQLLKKRMKL SSLDVFIIGA VAKAIATTVT YPLQTVQSIL RFGRHRLNPE NRTLGSLRNI LYLLHQRVRR FGIMGLYKGL EAKLLQTVLT AALMFLVYEK LTAATFTVMG LKRAHQH
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SLC25A17 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Solute Carrier Family 25 (Mitochondrial Carrier, Peroxisomal Membrane Protein, 34kDa), Member 17 (SLC25A17)
    Synonyms
    34kDa, 47kDa, PMP34, Pmp47, solute carrier family 25 (mitochondrial carrier, peroxisomal membrane protein), member 17, solute carrier family 25 member 17, Slc25a17, SLC25A17
    Background
    Peroxisomal transporter for multiple cofactors like coenzyme A (CoA), flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN) and nucleotide adenosine monophosphate (AMP), and to a lesser extend for nicotinamide adenine dinucleotide (NAD(+)), adenosine diphosphate (ADP) and adenosine 3',5'-diphosphate (PAP). May catalyze the transport of free CoA, FAD and NAD(+) from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. Inhibited by pyridoxal 5'-phosphate and bathophenanthroline in vitro. {ECO:0000269|PubMed:12445829, ECO:0000269|PubMed:22185573}.
    Molecular Weight
    35.7 kDa Including tag.
    UniProt
    O43808
    Pathways
    Monocarboxylic Acid Catabolic Process
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