Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

GLP2R Protein (AA 1-553) (rho-1D4 tag)

Crystallography grade GLP2R Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Catalog No. ABIN3111371
  • Target See all GLP2R Proteins
    GLP2R (Glucagon-Like Peptide 2 Receptor (GLP2R))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-553
    Origin
    • 7
    • 4
    • 1
    Human
    Source
    • 3
    • 3
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This GLP2R protein is labelled with rho-1D4 tag.
    Application
    Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MKLGSSRAGP GRGSAGLLPG VHELPMGIPA PWGTSPLSFH RKCSLWAPGR PFLTLVLLVS IKQVTGSLLE ETTRKWAQYK QACLRDLLKE PSGIFCNGTF DQYVCWPHSS PGNVSVPCPS YLPWWSEESS GRAYRHCLAQ GTWQTIENAT DIWQDDSECS ENHSFKQNVD RYALLSTLQL MYTVGYSFSL ISLFLALTLL LFLRKLHCTR NYIHMNLFAS FILRTLAVLV KDVVFYNSYS KRPDNENGWM SYLSEMSTSC RSVQVLLHYF VGANYLWLLV EGLYLHTLLE PTVLPERRLW PRYLLLGWAF PVLFVVPWGF ARAHLENTGC WTTNGNKKIW WIIRGPMMLC VTVNFFIFLK ILKLLISKLK AHQMCFRDYK YRLAKSTLVL IPLLGVHEIL FSFITDDQVE GFAKLIRLFI QLTLSSFHGF LVALQYGFAN GEVKAELRKY WVRFLLARHS GCRACVLGKD FRFLGKCPKK LSEGDGAEKL RKLQPSLNSG RLLHLAMRGL GELGAQPQQD HARWPRGSSL SECSEGDVTM ANTMEEILEE SEI
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human GLP2R Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product GLP2R Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    GLP2R (Glucagon-Like Peptide 2 Receptor (GLP2R))
    Alternative Name
    GLP2R (GLP2R Products)
    Synonyms
    9530092J08Rik Protein, GLP-2 Protein, glucagon like peptide 2 receptor Protein, glucagon-like peptide 2 receptor Protein, GLP2R Protein, Glp2r Protein
    Background
    This is a receptor for glucagon-like peptide 2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
    Molecular Weight
    64.2 kDa Including tag.
    UniProt
    O95838
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Feeding Behaviour
You are here:
Support