ATP2B1 Protein (AA 2-1258) (rho-1D4 tag)
-
- Target See all ATP2B1 Proteins
- ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-1258
-
Origin
- Human
-
Source
- Insect Cells
- Purification tag / Conjugate
- This ATP2B1 protein is labelled with rho-1D4 tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
-
GDMANNSVAY SGVKNSLKEA NHDGDFGITL AELRALMELR STDALRKIQE SYGDVYGICT KLKTSPNEGL SGNPADLERR EAVFGKNFIP PKKPKTFLQL VWEALQDVTL IILEIAAIVS LGLSFYQPPE GDNALCGEVS VGEEEGEGET GWIEGAAILL SVVCVVLVTA FNDWSKEKQF RGLQSRIEQE QKFTVIRGGQ VIQIPVADIT VGDIAQVKYG DLLPADGILI QGNDLKIDES SLTGESDHVK KSLDKDPLLL SGTHVMEGSG RMVVTAVGVN SQTGIIFTLL GAGGEEEEKK DEKKKEKKNK KQDGAIENRN KAKAQDGAAM EMQPLKSEEG GDGDEKDKKK ANLPKKEKSV LQGKLTKLAV QIGKAGLLMS AITVIILVLY FVIDTFWVQK RPWLAECTPI YIQYFVKFFI IGVTVLVVAV PEGLPLAVTI SLAYSVKKMM KDNNLVRHLD ACETMGNATA ICSDKTGTLT MNRMTVVQAY INEKHYKKVP EPEAIPPNIL SYLVTGISVN CAYTSKILPP EKEGGLPRHV GNKTECALLG LLLDLKRDYQ DVRNEIPEEA LYKVYTFNSV RKSMSTVLKN SDGSYRIFSK GASEIILKKC FKILSANGEA KVFRPRDRDD IVKTVIEPMA SEGLRTICLA FRDFPAGEPE PEWDNENDIV TGLTCIAVVG IEDPVRPEVP DAIKKCQRAG ITVRMVTGDN INTARAIATK CGILHPGEDF LCLEGKDFNR RIRNEKGEIE QERIDKIWPK LRVLARSSPT DKHTLVKGII DSTVSDQRQV VAVTGDGTND GPALKKADVG FAMGIAGTDV AKEASDIILT DDNFTSIVKA VMWGRNVYDS ISKFLQFQLT VNVVAVIVAF TGACITQDSP LKAVQMLWVN LIMDTLASLA LATEPPTESL LLRKPYGRNK PLISRTMMKN ILGHAFYQLV VVFTLLFAGE KFFDIDSGRN APLHAPPSEH YTIVFNTFVL MQLFNEINAR KIHGERNVFE GIFNNAIFCT IVLGTFVVQI IIVQFGGKPF SCSELSIEQW LWSIFLGMGT LLWGQLISTI PTSRLKFLKE AGHGTQKEEI PEEELAEDVE EIDHAERELR RGQILWFRGL NRIQTQMDVV NAFQSGSSIQ GALRRQPSIA SQHHDVTNIS TPTHIRVVNA FRSSLYEGLE KPESRSSIHN FMTHPEFRIE DSEPHIPLID DTDAEDDAPT KRNSSPPPSP NKNNNAVDSG IHLTIEMNKS ATSSSPGSPL HSLETSL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Human ATP2B1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product ATP2B1 Protein
-
-
- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
-
In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
-
- Target
- ATP2B1 (ATPase, Ca++ Transporting, Plasma Membrane 1 (ATP2B1))
- Alternative Name
- ATP2B1 (ATP2B1 Products)
- Synonyms
- PMCA1 Protein, PMCA1kb Protein, PMCA Protein, 2810442I22Rik Protein, E130111D10Rik Protein, Pmca1 Protein, ATP2B1 Protein, DKFZp459A1127 Protein, atp2b1 Protein, Pmca1a Protein, Pmca1b Protein, Pmca1c Protein, pmca1 Protein, pmca1a Protein, sb:cb801 Protein, si:dkey-18o7.1 Protein, wu:fe47b06 Protein, wu:fi41d01 Protein, atp2b1b Protein, ATPase plasma membrane Ca2+ transporting 1 Protein, ATPase, Ca++ transporting, plasma membrane 1 Protein, plasma membrane calcium-transporting ATPase 2 Protein, ATPase, Ca++ transporting, plasma membrane 1a Protein, ATPase, Ca++ transporting, plasma membrane 1 L homeolog Protein, ATP2B1 Protein, Atp2b1 Protein, atp2b1 Protein, LOC100180580 Protein, atp2b1a Protein, atp2b1.L Protein
- Background
- This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
- Molecular Weight
- 139.8 kDa Including tag.
- UniProt
- P20020
- Pathways
- Ribonucleoside Biosynthetic Process
-