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ZFYVE16 Protein (AA 1-1539) (His tag)

Crystallography grade ZFYVE16 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3116035
  • Target See all ZFYVE16 Proteins
    ZFYVE16 (Zinc Finger, FYVE Domain Containing 16 (ZFYVE16))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1539
    Origin
    Human
    Source
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ZFYVE16 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MDSYFKAAVS DLDKLLDDFE QNPDEQDYLQ DVQNAYDSNH CSVSSELASS QRTSLLPKDQ ECVNSCASSE TSYGTNESSL NEKTLKGLTS IQNEKNVTGL DLLSSVDGGT SDEIQPLYMG RCSKPICDLI SDMGNLVHAT NSEEDIKKLL PDDFKSNADS LIGLDLSSVS DTPCVSSTDH DSDTVREQQN DISSELQNRE IGGIKELGIK VDTTLSDSYN YSGTENLKDK KIFNQLESIV DFNMSSALTR QSSKMFHAKD KLQHKSQPCG LLKDVGLVKE EVDVAVITAA ECLKEEGKTS ALTCSLPKNE DLCLNDSNSR DENFKLPDFS FQEDKTVIKQ SAQEDSKSLD LKDNDVIQDS SSALHVSSKD VPSSLSCLPA SGSMCGSLIE SKARGDFLPQ HEHKDNIQDA VTIHEEIQNS VVLGGEPFKE NDLLKQEKCK SILLQSLIEG MEDRKIDPDQ TVIRAESLDG GDTSSTVVES QEGLSGTHVP ESSDCCEGFI NTFSSNDMDG QDLDYFNIDE GAKSGPLISD AELDAFLTEQ YLQTTNIKSF EENVNDSKSQ MNQIDMKGLD DGNINNIYFN AEAGAIGESH GINIICEIVD KQNTIENGLS LGEKSTIPVQ QGLPTSKSEI TNQLSVSDIN SQSVGGARPK QLFSLPSRTR SSKDLNKPDV PDTIESEPST ADTVVPITCA IDSTADPQVS FNSNYIDIES NSEGGSSFVT ANEDSVPENT CKEGLVLGQK QPTWVPDSEA PNCMNCQVKF TFTKRRHHCR ACGKVFCGVC CNRKCKLQYL EKEARVCVVC YETISKAQAF ERMMSPTGSN LKSNHSDECT TVQPPQENQT SSIPSPATLP VSALKQPGVE GLCSKEQKRV WFADGILPNG EVADTTKLSS GSKRCSEDFS PLSPDVPMTV NTVDHSHSTT VEKPNNETGD ITRNEIIQSP ISQVPSVEKL SMNTGNEGLP TSGSFTLDDD VFAETEEPSS PTGVLVNSNL PIASISDYRL LCDINKYVCN KISLLPNDED SLPPLLVASG EKGSVPVVEE HPSHEQIILL LEGESFHPVT FVLNANLLVN VKFIFYSSDK YWYFSTNGLH GLGQAEIIIL LLCLPNEDTI PKDIFRLFIT IYKDALKGKY IENLDNITFT ESFLSSKDHG GFLFITPTFQ KLDDLSLPSN PFLCGILIQK LEIPWAKVFP MRLMLRLGAE YKAYPAPLTS IRGRKPLFGE IGHTIMNLLV DLRNYQYTLH NIDQLLIHME MGKSCIKIPR KKYSDVMKVL NSSNEHVISI GASFSTEADS HLVCIQNDGI YETQANSATG HPRKVTGASF VVFNGALKTS SGFLAKSSIV EDGLMVQITP ETMNGLRLAL REQKDFKITC GKVDAVDLRE YVDICWVDAE EKGNKGVISS VDGISLQGFP SEKIKLEADF ETDEKIVKCT EVFYFLKDQD LSILSTSYQF AKEIAMACSA ALCPHLKTLK SNGMNKIGLR VSIDTDMVEF QAGSEGQLLP QHYLNDLDSA LIPVIHGGTS NSSLPLEIEL VFFIIEHLF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ZFYVE16 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ZFYVE16 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ZFYVE16 (Zinc Finger, FYVE Domain Containing 16 (ZFYVE16))
    Alternative Name
    ZFYVE16 (ZFYVE16 Products)
    Synonyms
    RGD1564784 Protein, ZFYVE16 Protein, ENDOFIN Protein, PPP1R69 Protein, AI035632 Protein, B130024H06Rik Protein, B130031L15 Protein, mKIAA0305 Protein, zinc finger FYVE-type containing 16 Protein, zinc finger, FYVE domain containing 16 Protein, Zfyve16 Protein, ZFYVE16 Protein, zfyve16 Protein
    Background
    May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes. {ECO:0000269|PubMed:11546807, ECO:0000269|PubMed:14613930}.
    Molecular Weight
    169.9 kDa Including tag.
    UniProt
    Q7Z3T8
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