FAM178A Protein (AA 1-1173) (Strep Tag)

Catalog No. ABIN3116361

Product Details

Target: FAM178A (Family with Sequence Similarity 178, Member A (FAM178A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1173

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FAM178A protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MTRRCMPARP GFPSSPAPGS SPPRCHLRPG STAHAAAGKR TESPGDRKQS IIDFFKPASK QDRHMLDSPQ KSNIKYGGSR LSITGTEQFE RKLSSPKESK PKRVPPEKSP IIEAFMKGVK EHHEDHGIHE SRRPCLSLAS KYLAKGTNIY VPSSYHLPKE MKSLKKKHRS PERRKSLFIH ENNEKNDRDR GKTNADSKKQ TTVAEADIFN NSSRSLSSRS SLSRHHPEES PLGAKFQLSL ASYCRERELK RLRKEQMEQR INSENSFSEA SSLSLKSSIE RKYKPRQEQR KQNDIIPGKN NLSNVENGHL SRKRSSSDSW EPTSAGSKQN KFPEKRKRNS VDSDLKSTRE SMIPKARESF LEKRPDGPHQ KEKFIKHIAL KTPGDVLRLE DISKEPSDET DGSSAGLAPS NSGNSGHHST RNSDQIQVAG TKETKMQKPH LPLSQEKSAI KKASNLQKNK TASSTTKEKE TKLPLLSRVP SAGSSLVPLN AKNCALPVSK KDKERSSSKE CSGHSTESTK HKEHKAKTNK ADSNVSSGKI SGGPLRSEYG TPTKSPPAAL EVVPCIPSPA APSDKAPSEG ESSGNSNAGS SALKRKLRGD FDSDEESLGY NLDSDEEEET LKSLEEIMAL NFNQTPAATG KPPALSKGLR SQSSDYTGHV HPGTYTNTLE RLVKEMEDTQ RLDELQKQLQ EDIRQGRGIK SPIRIGEEDS TDDEDGLLEE HKEFLKKFSV TIDAIPDHHP GEEIFNFLNS GKIFNQYTLD LRDSGFIGQS AVEKLILKSG KTDQIFLTTQ GFLTSAYHYV QCPVPVLKWL FRMMSVHTDC IVSVQILSTL MEITIRNDTF SDSPVWPWIP SLSDVAAVFF NMGIDFRSLF PLENLQPDFN EDYLVSETQT TSRGKESEDS SYKPIFSTLP ETNILNVVKF LGLCTSIHPE GYQDREIMLL ILMLFKMSLE KQLKQIPLVD FQSLLINLMK NIRDWNTKVP ELCLGINELS SHPHNLLWLV QLVPNWTSRG RQLRQCLSLV IISKLLDEKH EDVPNASNLQ VSVLHRYLVQ MKPSDLLKKM VLKKKAEQPD GIIDDSLHLE LEKQAYYLTY ILLHLVGEVS CSHSFSSGQR KHFVLLCGAL EKHVKCDIRE DARLFYRTKV KDLVARIHGK WQEIIQNCRP TQGQLHDFWV PDS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FAM178A (Family with Sequence Similarity 178, Member A (FAM178A))

Alternative Name: SLF2 (FAM178A Products)

Synonyms: C10orf6 Protein, 3632432H19 Protein, 6030443O07Rik Protein, AU014917 Protein, FAM178A Protein, SMC5-SMC6 complex localization factor 2 Protein, SLF2 Protein, Slf2 Protein

Background: SMC5-SMC6 complex localization factor protein 2 (Smc5/6 localization factor 1),FUNCTION: Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions (PubMed:25931565). Plays a role in SMC5-SMC6 complex recruitment for viral restriction. Forms a complex with SIMC1 and this complex is required to recruit SMC5-SMC6 complex to PML nuclear bodies and sites of viral replication (PubMed:36373674). {ECO:0000269|PubMed:25931565, ECO:0000269|PubMed:36373674}.

Molecular Weight: 131.9 kDa

UniProt: Q8IX21