GLMN Protein (AA 2-594) (His tag)

Catalog No. ABIN3117154

Product Details

Target: GLMN (Glomulin, FKBP Associated Protein (GLMN))

Protein Type: Recombinant

Protein Characteristics: AA 2-594

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This GLMN protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: AVEELQSIIK RCQILEEQDF KEEDFGLFQL AGQRCIEEGH TDQLLEIIQN EKNKVIIKNM GWNLVGPVVR CLLCKDKEDS KRKVYFLIFD LLVKLCNPKE LLLGLLELIE EPSGKQISQS ILLLLQPLQT VIQKLHNKAY SIGLALSTLW NQLSLLPVPY SKEQIQMDDY GLCQCCKALI EFTKPFVEEV IDNKENSLEN EKLKDELLKF CFKSLKCPLL TAQFFEQSEE GGNDPFRYFA SEIIGFLSAI GHPFPKMIFN HGRKKRTWNY LEFEEEENKQ LADSMASLAY LVFVQGIHID QLPMVLSPLY LLQFNMGHIE VFLQRTEESV ISKGLELLEN SLLRIEDNSL LYQYLEIKSF LTVPQGLVKV MTLCPIETLR KKSLAMLQLY INKLDSQGKY TLFRCLLNTS NHSGVEAFII QNIKNQIDMS LKRTRNNKWF TGPQLISLLD LVLFLPEGAE TDLLQNSDRI MASLNLLRYL VIKDNENDNQ TGLWTELGNI ENNFLKPLHI GLNMSKAHYE AEIKNSQEAQ KSKDLCSITV SGEEIPNMPP EMQLKVLHSA LFTFDLIESV LARVEELIEI KTKSTSEENI GIK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GLMN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GLMN (Glomulin, FKBP Associated Protein (GLMN))

Alternative Name: GLMN (GLMN Products)

Synonyms: FAP Protein, FAP48 Protein, FAP68 Protein, FKBPAP Protein, GLML Protein, GVM Protein, VMGLOM Protein, 9330160J16Rik Protein, AW227515 Protein, Fap48 Protein, Fap68 Protein, MGC69174 Protein, GLMN Protein, zgc:194957 Protein, glmnl Protein, zgc:101567 Protein, glomulin, FKBP associated protein Protein, glomulin, FKBP associated protein L homeolog Protein, glomulin, FKBP associated protein b Protein, glomulin, FKBP associated protein a Protein, GLMN Protein, Glmn Protein, glmn.L Protein, glmn Protein, glmnb Protein, glmna Protein

Background: Essential for normal development of the vasculature. May represent a naturally occurring ligand of the immunophilins FKBP59 and FKBP12. May function as an membrane anchoring protein. Isoform 1 may stimulate the p70S6K pathway. Isoform 2 may inhibit cell proliferation and increase IL2 production. {ECO:0000269|PubMed:11571281, ECO:0000269|PubMed:12604780}.

Molecular Weight: 69.0 kDa Including tag.

UniProt: Q92990

Pathways: Tube Formation