DISP1 Protein (AA 1-1524) (Strep Tag)

Catalog No. ABIN3117301

Product Details

Target: DISP1 (Dispatched Homolog 1 (DISP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1524

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DISP1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MAMSNGNNDF VVLSNSSIAT SAANPSPLTP CDGDHAAQQL TPKEATRTKV SPNGCLQLNG TVKSSFLPLD NQRMPQMLPQ CCHPCPYHHP LTSHSSHQEC HPEAGPAAPS ALASCCMQPH SEYSASLCPN HSPVYQTTCC LQPSPSFCLH HPWPDHFQHQ PVQQHIANIR PSRPFKLPKS YAALIADWPV VVLGMCTMFI VVCALVGVLV PELPDFSDPL LGFEPRGTAI GQRLVTWNNM VKNTGYKATL ANYPFKYADE QAKSHRDDRW SDDHYEREKR EVDWNFHKDS FFCDVPSDRY SRVVFTSSGG ETLWNLPAIK SMCNVDNSRI RSHPQFGDLC QRTTAASCCP SWTLGNYIAI LNNRSSCQKI VERDVSHTLK LLRTCAKHYQ NGTLGPDCWD MAARRKDQLK CTNVPRKCTK YNAVYQILHY LVDKDFMTPK TADYATPALK YSMLFSPTEK GESMMNIYLD NFENWNSSDG VTTITGIEFG IKHSLFQDYL LMDTVYPAIA IVIVLLVMCV YTKSMFITLM TMFAIISSLI VSYFLYRVVF HFEFFPFMNL TALIILVGIG ADDAFVLCDV WNYTKFDKPH AETSETVSIT LQHAALSMFV TSFTTAAAFY ANYVSNITAI RCFGVYAGTA ILVNYVLMVT WLPAVVVLHE RYLLNIFTCF KKPQQQIYDN KSCWTVACQK CHKVLFAISE ASRIFFEKVL PCIVIKFRYL WLFWFLALTV GGAYIVCINP KMKLPSLELS EFQVFRSSHP FERYDAEYKK LFMFERVHHG EELHMPITVI WGVSPEDNGN PLNPKSKGKL TLDSSFNIAS PASQAWILHF CQKLRNQTFF YQTDEQDFTS CFIETFKQWM ENQDCDEPAL YPCCSHWSFP YKQEIFELCI KRAIMELERS TGYHLDSKTP GPRFDINDTI RAVVLEFQST YLFTLAYEKM HQFYKEVDSW ISSELSSAPE GLSNGWFVSN LEFYDLQDSL SDGTLIAMGL SVAVAFSVML LTTWNIIISL YAIISIAGTI FVTVGSLVLL GWELNVLESV TISVAVGLSV DFAVHYGVAY RLAPDPDREG KVIFSLSRVG SAMAMAALTT FVAGAMMMPS TVLAYTQLGT FMMLIMCISW AFATFFFQCM CRCLGPQGTC GQIPLPKKLQ CSAFSHALST SPSDKGQSKT HTINAYHLDP RGPKSELEHE FYELEPLASH SCTAPEKTTY EETHICSEFF NSQAKNLGMP VHAAYNSELS KSTESDAGSA LLQPPLEQHT VCHFFSLNQR CSCPDAYKHL NYGPHSCQQM GDCLCHQCSP TTSSFVQIQN GVAPLKATHQ AVEGFVHPIT HIHHCPCLQG RVKPAGMQNS LPRNFFLHPV QHIQAQEKIG KTNVHSLQRS IEEHLPKMAE PSSFVCRSTG SLLKTCCDPE NKQRELCKNR DVSNLESSGG TENKAGGKVE LSLSQTDASV NSEHFNQNEP KVLFNHLMGE AGCRSCPNNS QSCGRIVRVK CNSVDCQMPN MEANVPAVLT HSELSGESLL IKTL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DISP1 (Dispatched Homolog 1 (DISP1))

Alternative Name: DISP1 (DISP1 Products)

Synonyms: DISP1 Protein, DISPA Protein, 1190008H24Rik Protein, DispA Protein, con Protein, zgc:111866 Protein, dispatched Protein, dispatched RND transporter family member 1 Protein, dispatched homolog 1 (Drosophila) Protein, CpipJ_CPIJ008642 Protein, DISP1 Protein, Disp1 Protein, disp1 Protein

Background: Protein dispatched homolog 1,FUNCTION: Functions in hedgehog (Hh) signaling. Regulates the release and extracellular accumulation of cholesterol-modified hedgehog proteins and is hence required for effective production of the Hh signal (By similarity). Synergizes with SCUBE2 to cause an increase in SHH secretion (PubMed:22902404). {ECO:0000250|UniProtKB:Q3TDN0, ECO:0000269|PubMed:22902404}.

Molecular Weight: 170.9 kDa

UniProt: Q96F81

Pathways: Hedgehog Signaling