SENP5 Protein (AA 1-755) (His tag)
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- Target See all SENP5 Proteins
- SENP5 (SUMO1/sentrin Specific Peptidase 5 (SENP5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-755
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This SENP5 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MKKQRKILWR KGIHLAFSEK WNTGFGGFKK FYFHQHLCIL KAKLGRPVTW NRQLRHFQGR KKALQIQKTW IKDEPLCAKT KFNVATQNVS TLSSKVKRKD AKHFISSSKT LLRLQAEKLL SSAKNSDHEY CREKNLLKAV TDFPSNSALG QANGHRPRTD PQPSDFPMKF NGESQSPGES GTIVVTLNNH KRKGFCYGCC QGPEHHRNGG PLIPKKFQLN QHRRIKLSPL MMYEKLSMIR FRYRILRSQH FRTKSKVCKL RKAQRSWVQK VTGDHQETRR ENGEGGSCSP FPSPEPKDPS CRHQPYFPDM DSSAVVKGTN SHVPDCHTKG SSFLGKELSL DEAFPDQQNG SATNAWDQSS CSSPKWECTE LIHDIPLPEH RSNTMFISET EREIMTLGQE NQTSSVSDDR VKLSVSGADT SVSSVDGPVS QKAVQNENSY QMEEDGSLKQ SILSSELLDH PYCKSPLEAP LVCSGLKLEN QVGGGKNSQK ASPVDDEQLS VCLSGFLDEV MKKYGSLVPL SEKEVLGRLK DVFNEDFSNR KPFINREITN YRARHQKCNF RIFYNKHMLD MDDLATLDGQ NWLNDQVINM YGELIMDAVP DKVHFFNSFF HRQLVTKGYN GVKRWTKKVD LFKKSLLLIP IHLEVHWSLI TVTLSNRIIS FYDSQGIHFK FCVENIRKYL LTEAREKNRP EFLQGWQTAV TKCIPQQKND SDCGVFVLQY CKCLALEQPF QFSQEDMPRV RKRIYKELCE CRLMD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SENP5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SENP5 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SENP5 (SUMO1/sentrin Specific Peptidase 5 (SENP5))
- Alternative Name
- SENP5 (SENP5 Products)
- Synonyms
- 6230429P13Rik Protein, A730063F07Rik Protein, AI851888 Protein, BB189556 Protein, SMT3IP3 Protein, SENP5 Protein, Senp5 Protein, senp5 Protein, SUMO1/sentrin specific peptidase 5 Protein, SUMO/sentrin specific peptidase 5 Protein, SUMO1/sentrin specific peptidase 5 L homeolog Protein, SENP5 Protein, Senp5 Protein, senp5 Protein, senp5.L Protein
- Background
- Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division. {ECO:0000269|PubMed:16608850, ECO:0000269|PubMed:16738315}.
- Molecular Weight
- 87.6 kDa Including tag.
- UniProt
- Q96HI0
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