ADAMTS9 Protein (AA 288-1935) (His tag)

Catalog No. ABIN3118794

Product Details

Target: ADAMTS9 (ADAM Metallopeptidase with thrombospondin Type 1 Motif, 9 (ADAMTS9))

Protein Type: Recombinant

Protein Characteristics: AA 288-1935

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This ADAMTS9 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequence: FLSYPRFVEV LVVADNRMVS YHGENLQHYI LTLMSIVASI YKDPSIGNLI NIVIVNLIVI HNEQDGPSIS FNAQTTLKNF CQWQHSKNSP GGIHHDTAVL LTRQDICRAH DKCDTLGLAE LGTICDPYRS CSISEDSGLS TAFTIAHELG HVFNMPHDDN NKCKEEGVKS PQHVMAPTLN FYTNPWMWSK CSRKYITEFL DTGYGECLLN EPESRPYPLP VQLPGILYNV NKQCELIFGP GSQVCPYMMQ CRRLWCNNVN GVHKGCRTQH TPWADGTECE PGKHCKYGFC VPKEMDVPVT DGSWGSWSPF GTCSRTCGGG IKTAIRECNR PEPKNGGKYC VGRRMKFKSC NTEPCLKQKR DFRDEQCAHF DGKHFNINGL LPNVRWVPKY SGILMKDRCK LFCRVAGNTA YYQLRDRVID GTPCGQDTND ICVQGLCRQA GCDHVLNSKA RRDKCGVCGG DNSSCKTVAG TFNTVHYGYN TVVRIPAGAT NIDVRQHSFS GETDDDNYLA LSSSKGEFLL NGNFVVTMAK REIRIGNAVV EYSGSETAVE RINSTDRIEQ ELLLQVLSVG KLYNPDVRYS FNIPIEDKPQ QFYWNSHGPW QACSKPCQGE RKRKLVCTRE SDQLTVSDQR CDRLPQPGHI TEPCGTDCDL RWHVASRSEC SAQCGLGYRT LDIYCAKYSR LDGKTEKVDD GFCSSHPKPS NREKCSGECN TGGWRYSAWT ECSKSCDGGT QRRRAICVNT RNDVLDDSKC THQEKVTIQR CSEFPCPQWK SGDWSECLVT CGKGHKHRQV WCQFGEDRLN DRMCDPETKP TSMQTCQQPE CASWQAGPWG QCSVTCGQGY QLRAVKCIIG TYMSVVDDND CNAATRPTDT QDCELPSCHP PPAAPETRRS TYSAPRTQWR FGSWTPCSAT CGKGTRMRYV SCRDENGSVA DESACATLPR PVAKEECSVT PCGQWKALDW SSCSVTCGQG RATRQVMCVN YSDHVIDRSE CDQDYIPETD QDCSMSPCPQ RTPDSGLAQH PFQNEDYRPR SASPSRTHVL GGNQWRTGPW GACSSTCAGG SQRRVVVCQD ENGYTANDCV ERIKPDEQRA CESGPCPQWA YGNWGECTKL CGGGIRTRLV VCQRSNGERF PDLSCEILDK PPDREQCNTH ACPHDAAWST GPWSSCSVSC GRGHKQRNVY CMAKDGSHLE SDYCKHLAKP HGHRKCRGGR CPKWKAGAWS QCSVSCGRGV QQRHVGCQIG THKIARETEC NPYTRPESER DCQGPRCPLY TWRAEEWQEC TKTCGEGSRY RKVVCVDDNK NEVHGARCDV SKRPVDRESC SLQPCEYVWI TGEWSECSVT CGKGYKQRLV SCSEIYTGKE NYEYSYQTTI NCPGTQPPSV HPCYLRDCPV SATWRVGNWG SCSVSCGVGV MQRSVQCLTN EDQPSHLCHT DLKPEERKTC RNVYNCELPQ NCKEVKRLKG ASEDGEYFLM IRGKLLKIFC AGMHSDHPKE YVTLVHGDSE NFSEVYGHRL HNPTECPYNG SRRDDCQCRK DYTAAGFSSF QKIRIDLTSM QIITTDLQFA RTSEGHPVPF ATAGDCYSAA KCPQGRFSIN LYGTGLSLTE SARWISQGNY AVSDIKKSPD GTRVVGKCGG YCGKCTPSSG TGLEVRVL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ADAMTS9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ADAMTS9 (ADAM Metallopeptidase with thrombospondin Type 1 Motif, 9 (ADAMTS9))

Alternative Name: ADAMTS9 (ADAMTS9 Products)

Synonyms: 1810011L16Rik Protein, 8430403M15Rik Protein, AW743315 Protein, E030027K14Rik Protein, ADAM metallopeptidase with thrombospondin type 1 motif 9 Protein, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 Protein, ADAM metallopeptidase with thrombospondin type 1 motif, 9 Protein, ADAMTS9 Protein, Adamts9 Protein

Background: Cleaves the large aggregating proteoglycans, aggrecan and versican. Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos. {ECO:0000269|PubMed:12514189, ECO:0000269|PubMed:22419820}.

Molecular Weight: 185.1 kDa Including tag.

UniProt: Q9P2N4