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SIX4 Protein (AA 2-781) (His tag)

Crystallography grade SIX4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3118954
  • Target See all SIX4 Proteins
    SIX4 (SIX Homeobox 4 (SIX4))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-781
    Origin
    • 3
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This SIX4 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    SSSSPTGQIA SAADIKQENG MESASEGQEA HREVAGGAAV GLSPPAPAPF PLEPGDAATA AARVSGEEGA VAAAAAGAAA DQVQLHSELL GRHHHAAAAA AQTPLAFSPD HVACVCEALQ QGGNLDRLAR FLWSLPQSDL LRGNESLLKA RALVAFHQGI YPELYSILES HSFESANHPL LQQLWYKARY TEAERARGRP LGAVDKYRLR RKFPLPRTIW DGEETVYCFK EKSRNALKEL YKQNRYPSPA EKRHLAKITG LSLTQVSNWF KNRRQRDRNP SETQSKSESD GNPSTEDESS KGHEDLSPHP LSSSSDGITN LSLSSHMEPV YMQQIGNAKI SLSSSGVLLN GSLVPASTSP VFLNGNSFIQ GPSGVILNGL NVGNTQAVAL NPPKMSSNIV SNGISMTDIL GSTSQDVKEF KVLQSSANSA TTTSYSPSVP VSFPGLIPST EVKREGIQTV ASQDGGSVVT FTTPVQINQY GIVQIPNSGA NSQFLNGSIG FSPLQLPPVS VAASQGNISV SSSTSDGSTF TSESTTVQQG KVFLSSLAPS AVVYTVPNTG QTIGSVKQEG LERSLVFSQL MPVNQNAQVN ANLSSENISG SGLHPLASSL VNVSPTHNFS LSPSTLLNPT ELNRDIADSQ PMSAPVASKS TVTSVSNTNY ATLQNCSLIT GQDLLSVPMT QAALGEIVPT AEDQVGHPSP AVHQDFVQEH RLVLQSVANM KENFLSNSES KATSSLMMLD SKSKYVLDGM VDTVCEDLET DKKELAKLQT VQLDEDMQDL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SIX4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SIX4 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SIX4 (SIX Homeobox 4 (SIX4))
    Alternative Name
    SIX4 (SIX4 Products)
    Synonyms
    AREC3 Protein, AI047561 Protein, TrexBF Protein, fk50f02 Protein, six4.2 Protein, six4b Protein, wu:fk50f02 Protein, SIX homeobox 4 Protein, sine oculis-related homeobox 4 Protein, SIX homeobox 4a Protein, SIX4 Protein, Six4 Protein, six4a Protein
    Background
    Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a DNA sequence on these target genes and is involved in processes like cell differentiation, cell migration and cell survival. Transactivates gene expression by binding a 5'-[CAT]A[CT][CT][CTG]GA[GAT]-3' motif present in the Trex site and a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 site of the muscle-specific genes enhancer. Acts cooperatively with EYA proteins to transactivate their target genes through interaction and nuclear translocation of EYA protein. Acts synergistically with SIX1 to regulate target genes involved in formation of various organs, including muscle, kidney, gonad, ganglia, olfactory epithelium and cranial skeleton. Plays a role in several important steps of muscle development. Controls the genesis of hypaxial myogenic progenitors in the dermomyotome by transactivating PAX3 and the delamination and migration of the hypaxial precursors from the ventral lip to the limb buds through the transactivation of PAX3, MET and LBX1. Controls myoblast determination by transactivating MYF5, MYOD1 and MYF6. Controls somitic differentiation in myocyte through MYOG transactivation. Plays a role in synaptogenesis and sarcomere organization by participating in myofiber specialization during embryogenesis by activating fast muscle program in the primary myotome resulting in an up-regulation of fast muscle genes, including ATP2A1, MYL1 and TNNT3. Simultaneously, is also able to activate inhibitors of slow muscle genes, such as SOX6, HRASLS, and HDAC4, thereby restricting the activation of the slow muscle genes. During muscle regeneration, negatively regulates differentiation of muscle satellite cells through down-regulation of MYOG expression. During kidney development regulates the early stages of metanephros development and ureteric bud formation through regulation of GDNF, SALL1, PAX8 and PAX2 expression. Plays a role in gonad development by regulating both testis determination and size determination. In gonadal sex determination, transactivates ZFPM2 by binding a MEF3 consensus sequence, resulting in SRY up-regulation. In gonadal size determination, transactivates NR5A1 by binding a MEF3 consensus sequence resulting in gonadal precursor cell formation regulation. During olfactory development mediates the specification and patterning of olfactory placode through fibroblast growth factor and BMP4 signaling pathways and also regulates epithelial cell proliferation during placode formation. Promotes survival of sensory neurons during early trigeminal gangliogenesis. In the developing dorsal root ganglia, up-regulates SLC12A2 transcription. Regulates early thymus/parathyroid organogenesis through regulation of GCM2 and FOXN1 expression. Forms gustatory papillae during development of the tongue. Also plays a role during embryonic cranial skeleton morphogenesis. {ECO:0000250|UniProtKB:Q61321}.
    Molecular Weight
    83.8 kDa Including tag.
    UniProt
    Q9UIU6
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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