SNX14 Protein (AA 1-946) (rho-1D4 tag)
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- Target See all SNX14 Proteins
- SNX14 (Sorting Nexin 14 (SNX14))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-946
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This SNX14 protein is labelled with rho-1D4 tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MVPWVRTMGQ KLKQRLRLDV GREICRQYPL FCFLLLCLSA ASLLLNRYIH ILMIFWSFVA GVVTFYCSLG PDSLLPNIFF TIKYKPKQLG LQELFPQGHS CAVCGKVKCK RHRPSLLLEN YQPWLDLKIS SKVDASLSEV LELVLENFVY PWYRDVTDDE SFVDELRITL RFFASVLIRR IHKVDIPSII TKKLLKAAMK HIEVIVKARQ KVKNTEFLQQ AALEEYGPEL HVALRSRRDE LHYLRKLTEL LFPYILPPKA TDCRSLTLLI REILSGSVFL PSLDFLADPD TVNHLLIIFI DDSPPEKATE PASPLVPFLQ KFAEPRNKKP SVLKLELKQI REQQDLLFRF MNFLKQEGAV HVLQFCLTVE EFNDRILRPE LSNDEMLSLH EELQKIYKTY CLDESIDKIR FDPFIVEEIQ RIAEGPYIDV VKLQTMRCLF EAYEHVLSLL ENVFTPMFCH SDEYFRQLLR GAESPTRNSK LNRGSLSLDD FRNTQKRGES FGISRIGSKI KGVFKSTTME GAMLPNYGVA EGEDDFIEEG IVVMEDDSPV EAVSTPNTPR NLAAWKISIP YVDFFEDPSS ERKEKKERIP VFCIDVERND RRAVGHEPEH WSVYRRYLEF YVLESKLTEF HGAFPDAQLP SKRIIGPKNY EFLKSKREEF QEYLQKLLQH PELSNSQLLA DFLSPNGGET QFLDKILPDV NLGKIIKSVP GKLMKEKGQH LEPFIMNFIN SCESPKPKPS RPELTILSPT SENNKKLFND LFKNNANRAE NTERKQNQNY FMEVMTVEGV YDYLMYVGRV VFQVPDWLHH LLMGTRILFK NTLEMYTDYY LQCKLEQLFQ EHRLVSLITL LRDAIFCENT EPRSLQDKQK GAKQTFEEMM NYIPDLLVKC IGEETKYESI RLLFDGLQQP VLNKQLTYVL LDIVIQELFP ELNKVQKEVT SVTSWM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SNX14 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin-free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SNX14 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SNX14 (Sorting Nexin 14 (SNX14))
- Alternative Name
- SNX14 (SNX14 Products)
- Synonyms
- SNX14 Protein, si:ch211-241p10.3 Protein, snx14 Protein, MGC146224 Protein, RGS-PX2 Protein, B830022K16 Protein, C330035N22Rik Protein, YR-14 Protein, sorting nexin 14 Protein, sorting nexin 14 L homeolog Protein, Snx14 Protein, SNX14 Protein, snx14.L Protein, snx14 Protein
- Background
- Plays a role in maintaining normal neuronal excitability and synaptic transmission. May be involved in several stages of intracellular trafficking (By similarity). Required for autophagosome clearance, possibly by mediating the fusion of lysosomes with autophagosomes (Probable). Binds phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2), a key component of late endosomes/lysosomes (PubMed:25848753). Does not bind phosphatidylinositol 3-phosphate (PtdIns(3P)) (PubMed:25848753, PubMed:25148684). {ECO:0000250|UniProtKB:Q8BHY8, ECO:0000269|PubMed:25148684, ECO:0000269|PubMed:25848753, ECO:0000305|PubMed:25848753}.
- Molecular Weight
- 111.4 kDa Including tag.
- UniProt
- Q9Y5W7
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling
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