AADAT Protein (AA 30-425) (His tag)

Catalog No. ABIN3120175

Product Details

Target: AADAT (Aminoadipate Aminotransferase (AADAT))

Protein Type: Recombinant

Protein Characteristics: AA 30-425

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This AADAT protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: MHHHHHHPKTLISLAPG SPNPSMFPFK SAAFTVENGS TIRFEDDLIK RALQYSPSYG IPELLSWLKQ FQVKLHNPPT VNYPPNQGQM DLCITSGCQD GLCKAFEMLI NPGDTILVNE PLFPGTLYAM KPLGCNIINV PSDEHGIIPE GLKKILSQWK PEDSKDPTKK TPKFLYTVPN GNNPTGNSLT GDRKKEIYEL ARKYDFLIIE DDPYYFLQFS KPWEPTFLSM DVDGRVIRAD TFSKTVSSGL RVGFMTGPKT LIQNIVLHTQ VSSVHACTLS QLMILQLLHQ WGEEGFLAHI DRTIDFYKNQ RDSILAAADK WLRGLAEWHV PKAGMFLWIK VKGISDTKQL IEEKAIEREV LLVPGNGFFI DGSAPTSFFR ASFSLATPAQ MDTAFQRLAQ LIKESL
N-terminal His-tag

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Aadat Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using DETA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE.
Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in solution (20 mM Hepes, pH 7.5)

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: AADAT (Aminoadipate Aminotransferase (AADAT))

Alternative Name: Aadat (AADAT Products)

Synonyms: KAT2 Protein, KATII Protein, AI875679 Protein, Aadt Protein, Kat2 Protein, mKat-2 Protein, MGC80030 Protein, kat2 Protein, katii Protein, aminoadipate aminotransferase Protein, aminoadipate aminotransferase S homeolog Protein, AADAT Protein, Aadat Protein, aadat.S Protein, aadat Protein

Background: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro) (By similarity). {ECO:0000250}.

Molecular Weight: 45.4 kDa Including tag.

UniProt: Q9WVM8