GIPC1 Protein (AA 1-333) (His tag)
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- Target See all GIPC1 Proteins
- GIPC1 (GIPC PDZ Domain Containing Family, Member 1 (GIPC1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-333
- Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GIPC1 protein is labelled with His tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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MPLGLGRRKK APPLVENEEA EPSRSGLGVG EPGPLGGSAA GESQMGLPPP PAALRPRLVF HTQLAHGSPT GRIEGFTNVK ELYGKIAEAF RLPAAEVMFC TLNTHKVDMD KLLGGQIGLE DFIFAHVKGQ RKEVEVFKSE EALGLTITDN GAGYAFIKRI KEGSVIDHIQ LISVGDMIEA INGQSLLGCR HYEVARLLKE LPRGRTFTLK LTEPRKAFDM ISQRSAGGHP GSGPQLGTGR GTLRLRSRGP ATVEDLPSAF EEKAIEKVDD LLESYMGIRD TELAATMVEL GKDKRNPDEL AEALDERLGD FAFPDEFVFD VWGAIGDAKV GRY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Gipc1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
- Top Product
- Discover our top product GIPC1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- GIPC1 (GIPC PDZ Domain Containing Family, Member 1 (GIPC1))
- Alternative Name
- Gipc1 (GIPC1 Products)
- Synonyms
- C19orf3 Protein, GIPC Protein, GLUT1CBP Protein, Hs.6454 Protein, IIP-1 Protein, NIP Protein, RGS19IP1 Protein, SEMCAP Protein, SYNECTIIN Protein, SYNECTIN Protein, TIP-2 Protein, XGIPC Protein, kermit Protein, rgs19ip1 Protein, gipc3 Protein, iip-1 Protein, Kermit Protein, semcap Protein, glut1cbp Protein, synectiin Protein, GIPC1 Protein, Glut1CIP Protein, Rgs19ip1 Protein, Semcap1 Protein, TaxIP2 Protein, Gipc Protein, Rgs19 Protein, GIPC PDZ domain containing family member 1 Protein, GIPC PDZ domain containing family member 1 L homeolog Protein, GIPC PDZ domain containing family, member 1 Protein, RGS-GAIP interacting protein GIPC Protein, PDZ domain-containing protein GIPC3 Protein, putative rgs-gaip interacting protein gipc Protein, GIPC1 Protein, gipc1.L Protein, gipc1 Protein, Gipc1 Protein, LOC692815 Protein, LOC5576629 Protein, Smp_170870 Protein
- Background
- Inhibits endothelial cell migartion (in vitro). May be involved in G protein-linked signaling (By similarity). {ECO:0000250}.
- Molecular Weight
- 37.1 kDa Including tag.
- UniProt
- Q9Z0G0
- Pathways
- Dicarboxylic Acid Transport
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