MTMR9 Protein (AA 1-545) (Strep Tag)

Catalog No. ABIN3120363

Product Details

Target: MTMR9 (Myotubularin Related Protein 9 (MTMR9))

Protein Type: Recombinant

Protein Characteristics: AA 1-545

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MTMR9 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MEFAELIKTP RVDNVVLHRP FYTAVEGTLC LTGHHLILSS RQDNTEELWL LHSNIDAIDK RFVGSLGTII IKCKDFRIIQ LDIPGMEECL NIASSIEALS TLDSVTLMYP FFYRPMFEVI EDGWHSFLPE QEFEFYSSAT SEWRLSYINK DFSICPSYPP TVIVPKSVDD EALRKVAAFR HGGRFPVLSY YHKKNGMVIM RSGQPLTGTN GRRCKEDEKL INATLRAGKR GYLIDTRSLN VAQQARAKGG GFEQEAHYPQ WRRIHKSIER YHVLQESLIK LVEACNEQTH NMDRWLGKLE ASNWLTHIKE ILTTACLAAQ CIDREGASVL IHGTEGTDST LQVTSLAQII LEPRSRTIRG FEALIEREWL QAGHPFQQRC AQSAYCSSKQ KWEAPVFLLF LDCVWQILRQ FPCSFEFNEH FLIMLFEHAY ASQFGTFLGN NESERCKLKL QQKTMSLWSW VNRPGELSKF TNPLFEANNL VIWPSVAPQS LQLWEGIFLR WSRSSKYLDE AYEEMVNIIE YNKELQAKVN VLRRQLAELE TEDGL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MTMR9 (Myotubularin Related Protein 9 (MTMR9))

Alternative Name: Mtmr9 (MTMR9 Products)

Synonyms: C8orf9 Protein, LIP-STYX Protein, MTMR8 Protein, 9430075G12Rik Protein, AA516943 Protein, AF073881 Protein, mMTMH3 Protein, MTMR9 Protein, si:dkey-155d18.2 Protein, MGC121706 Protein, MGC145907 Protein, MTMR9L Protein, myotubularin related protein 9 Protein, myotubularin related protein 9 L homeolog Protein, myotubularin related protein 9-like Protein, MTMR9 Protein, Mtmr9 Protein, mtmr9.L Protein, mtmr9 Protein

Background: Myotubularin-related protein 9 (Inactive phosphatidylinositol 3-phosphatase 9),FUNCTION: Acts as an adapter for myotubularin-related phosphatases (PubMed:12890864). Increases lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate, and MTMR6 binding affinity for phosphorylated phosphatidylinositols (By similarity). Positively regulates lipid phosphatase MTMR7 catalytic activity (PubMed:12890864). The formation of the MTMR6-MTMR9 complex, stabilizes both MTMR6 and MTMR9 protein levels (By similarity). Plays a role in the late stages of macropinocytosis possibly by regulating MTMR6-mediated dephosphorylation of phosphatidylinositol 3-phosphate in membrane ruffles (By similarity). Negatively regulates DNA damage-induced apoptosis, in part via its association with MTMR6 (By similarity). Does not bind mono-, di- and tri-phosphorylated phosphatidylinositols, phosphatidic acid and phosphatidylserine (By similarity). {ECO:0000250|UniProtKB:Q96QG7, ECO:0000269|PubMed:12890864}.

Molecular Weight: 62.9 kDa

UniProt: Q9Z2D0