PCK1 Protein (AA 1-622) (Strep Tag)

Catalog No. ABIN3120409

Product Details

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Protein Type: Recombinant

Protein Characteristics: AA 1-622

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PCK1 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MPPQLHNGLD FSAKVIQGSL DSLPQAVRKF VEGNAQLCQP EYIHICDGSE EEYGQLLAHM QEEGVIRKLK KYDNCWLALT DPRDVARIES KTVIITQEQR DTVPIPKTGL SQLGRWMSEE DFEKAFNARF PGCMKGRTMY VIPFSMGPLG SPLAKIGIEL TDSPYVVASM RIMTRMGISV LEALGDGEFI KCLHSVGCPL PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI ASRLAKEEGW LAEHMLILGI TNPEGKKKYL AAAFPSACGK TNLAMMNPSL PGWKVECVGD DIAWMKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT IQKNTIFTNV AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDAEPCA HPNSRFCTPA SQCPIIDPAW ESPEGVPIEG IIFGGRRPEG VPLVYEALSW QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM AHRPAAKLPK IFHVNWFRKD KDGKFLWPGF GENSRVLEWM FGRIEGEDSA KLTPIGYIPK ENALNLKGLG GVNVEELFGI SKEFWEKEVE EIDRYLEDQV NTDLPYEIER ELRALKQRIS QM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: Pck1 (PCK1 Products)

Synonyms: PEPCK-C Protein, PEPCK1 Protein, PEPCKC Protein, PEPCK Protein, PEPCK-M Protein, PEPCK2 Protein, 1810010O14Rik Protein, 9130022B02Rik Protein, cb856 Protein, cb924 Protein, fj93h11 Protein, zgc:63869 Protein, wu:fc51c05 Protein, wu:fj93h11 Protein, pepck-c Protein, pepck1 Protein, pepckc Protein, PCK1 Protein, PPCK1 Protein, AI265463 Protein, Pck-1 Protein, GTP Protein, PCK Protein, Pepck Protein, RATPEPCK Protein, Ppc1C Protein, PHOSPHOENOLPYRUVATE CARBOXYKINASE Protein, T28I19.150 Protein, T28I19_150 Protein, phosphoenolpyruvate carboxykinase 1 Protein, 143299_at Protein, CG10924 Protein, CG17725 Protein, Dmel\\CG17725 Protein, Dromel_CG17725_FBtr0086701_pepck_mORF Protein, dPEPCK Protein, pepck Protein, PEPC Protein, PEPCase Protein, ppc Protein, phosphoenolpyruvate carboxykinase 1 Protein, phosphoenolpyruvate carboxykinase 2, mitochondrial Protein, phosphoenolpyruvate carboxykinase 2 (mitochondrial) Protein, phosphoenolpyruvate carboxykinase 1 (soluble) Protein, phosphoenolpyruvate carboxykinase 1 S homeolog Protein, phosphoenolpyruvate carboxykinase, cytosolic [GTP] Protein, phosphoenolpyruvate carboxykinase 1, cytosolic Protein, phosphoenolpyruvate carboxylase 7 Protein, Phosphoenolpyruvate carboxykinase Protein, phosphoenolpyruvate carboxylase Protein, PCK1 Protein, PCK2 Protein, Pck2 Protein, pck1 Protein, pck1.S Protein, LOC100634531 Protein, Pck1 Protein, pep7 Protein, Pepck Protein, LOC107777405 Protein

Background: Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (PEPCK-C) (EC 4.1.1.32) (Serine-protein kinase PCK1) (EC 2.7.11.-),FUNCTION: Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed:11916968, PubMed:11792850, PubMed:30193097, PubMed:29230018). Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle (PubMed:30193097). At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:30193097). At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate (PubMed:30193097). Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up-regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis (PubMed:29230018). The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis (PubMed:29230018). In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor (By similarity). The protein kinase activity regulates lipogenesis: upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis-related genes (By similarity). {ECO:0000250|UniProtKB:P35558, ECO:0000269|PubMed:11792850, ECO:0000269|PubMed:11916968, ECO:0000269|PubMed:29230018, ECO:0000269|PubMed:30193097}.

Molecular Weight: 69.4 kDa

UniProt: Q9Z2V4

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis