ABCE1 Protein (AA 1-599) (His tag)
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- Target See all ABCE1 Proteins
- ABCE1 (ATP-Binding Cassette, Sub-Family E (OABP), Member 1 (ABCE1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-599
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This ABCE1 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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MADKLTRIAI VNHDKCKPKK CRQECKKSCP VVRMGKLCIE VTPQSKIAWI SETLCIGCGI CIKKCPFGAL SIVNLPSNLE KETTHRYCAN AFKLHRLPIP RPGEVLGLVG TNGIGKSTAL KILAGKQKPN LGKYDDPPDW QEILTYFRGS ELQNYFTKIL EDDLKAIIKP QYVDQIPKAA KGTVGSILDR KDETKTQAIV CQQLDLTHLK ERNVEDLSGG ELQRFACAVV CIQKADIFMF DEPSSYLDVK QRLKAAITIR SLINPDRYII VVEHDLSVLD YLSDFICCLY GVPSAYGVVT MPFSVREGIN IFLDGYVPTE NLRFRDASLV FKVAETANEE EVKKMCMYKY PGMKKKMGEF ELAIVAGEFT DSEIMVMLGE NGTGKTTFIR MLAGRLKPDE GGEVPVLNVS YKPQKISPKS TGSVRQLLHE KIRDAYTHPQ FVTDVMKPLQ IENIIDQEVQ TLSGGELQRV ALALCLGKPA DVYLIDEPSA YLDSEQRLMA ARVVKRFILH AKKTAFVVEH DFIMATYLAD RVIVFDGVPS KNTVANSPQT LLAGMNKFLS QLEITFRRDP NNYRPRINKL NSIKDVEQKK SGNYFFLDD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Abce1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product ABCE1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- ABCE1 (ATP-Binding Cassette, Sub-Family E (OABP), Member 1 (ABCE1))
- Alternative Name
- Abce1 (ABCE1 Products)
- Synonyms
- ABCE1 Protein, DDBDRAFT_0188931 Protein, DDBDRAFT_0191227 Protein, DDB_0188931 Protein, DDB_0191227 Protein, abce1 Protein, MGC69546 Protein, DKFZp469K1416 Protein, ABC38 Protein, OABP Protein, RLI Protein, RNASEL1 Protein, RNASELI Protein, RNS4I Protein, C79080 Protein, Oabp Protein, RNS41 Protein, Rnaseli Protein, wu:fb34c09 Protein, wu:fe47b01 Protein, wu:fi09g07 Protein, zgc:111906 Protein, zgc:56045 Protein, Rns4i Protein, ATP binding cassette subfamily E member 1 Protein, 4Fe-4S ferredoxin, iron-sulfur binding domain-containing protein Protein, ATP-binding cassette sub-family E member 1 Protein, RNAse L inhibitor Protein, RNase L inhibitor Protein, ribosome biogenesis/translation initiation ATPase RLI Protein, ATP-binding cassette, sub-family E (OABP), member 1 Protein, ATP binding cassette subfamily E member 1 S homeolog Protein, ABCE1 Protein, abcE1 Protein, LOC100184673 Protein, LOC100213121 Protein, abce1 Protein, TP04_0855 Protein, PVX_115370 Protein, TERMP_RS03360 Protein, Abce1 Protein, abce1.S Protein
- Background
- Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway (By similarity). {ECO:0000250, ECO:0000269|PubMed:10866653}.
- Molecular Weight
- 68.3 kDa Including tag.
- UniProt
- P61222
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