PPP3CA Protein (AA 2-521) (His tag)

Catalog No. ABIN3120872

Product Details

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Protein Type: Recombinant

Protein Characteristics: AA 2-521

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This PPP3CA protein is labelled with His tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)

Sequence: SEPKAIDPKL STTDRVVKAV PFPPSHRLTA KEVFDNDGKP RVDILKAHLM KEGRLEESVA LRIITEGASI LRQEKNLLDI DAPVTVCGDI HGQFFDLMKL FEVGGSPANT RYLFLGDYVD RGYFSIECVL YLWALKILYP KTLFLLRGNH ECRHLTEYFT FKQECKIKYS ERVYDACMDA FDCLPLAALM NQQFLCVHGG LSPEINTLDD IRKLDRFKEP PAYGPMCDIL WSDPLEDFGN EKTQEHFTHN TVRGCSYFYS YPAVCDFLQH NNLLSILRAH EAQDAGYRMY RKSQTTGFPS LITIFSAPNY LDVYNNKAAV LKYENNVMNI RQFNCSPHPY WLPNFMDVFT WSLPFVGEKV TEMLVNVLNI CSDDELGSEE DGFDGATAAA RKEVIRNKIR AIGKMARVFS VLREESESVL TLKGLTPTGM LPSGVLSGGK QTLQSATVEA IEADEAIKGF SPQHKITSFE EAKGLDRINE RMPPRRDAMP SDANLNSINK ALASETNGTD SNGSNSSNIQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ppp3ca Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PPP3CA (Protein Phosphatase 3, Catalytic Subunit, alpha Isoform (PPP3CA))

Alternative Name: Ppp3ca (PPP3CA Products)

Synonyms: CALN Protein, CALNA Protein, CALNA1 Protein, CCN1 Protein, CNA1 Protein, PPP2B Protein, 2900074D19Rik Protein, AI841391 Protein, AW413465 Protein, CN Protein, Caln Protein, Calna Protein, CnA Protein, Calna1 Protein, calcineurin Protein, caln Protein, calna Protein, calna1 Protein, ccn1 Protein, cna1 Protein, ppp2b Protein, fj46e08 Protein, si:dkeyp-79c2.2 Protein, wu:fj46e08 Protein, ppp3ca Protein, protein phosphatase 3 catalytic subunit alpha Protein, protein phosphatase 3, catalytic subunit, alpha isoform Protein, protein phosphatase 3, catalytic subunit, alpha isozyme Protein, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) Protein, protein phosphatase 3, catalytic subunit, alpha isozyme L homeolog Protein, serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Protein, PPP3CA Protein, Ppp3ca Protein, ppp3ca Protein, ppp3ca.L Protein, LOC100205420 Protein

Background: Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L and HSPB1 (By similarity). Dephosphorylates SSH1. {ECO:0000250, ECO:0000269|PubMed:1328240}.

Molecular Weight: 59.5 kDa Including tag.

UniProt: P63328

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Synaptic Membrane, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling