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TUBA1A Protein (AA 1-451) (His tag)

Crystallography grade Tuba1a Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3120882
  • Target See all TUBA1A (Tuba1a) Proteins
    TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-451
    Origin
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 8
    • 2
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This TUBA1A protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL IGQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRT IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE Y
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Tuba1a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product Tuba1a Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))
    Alternative Name
    Tuba1a (Tuba1a Products)
    Synonyms
    Tuba1 Protein, B-ALPHA-1 Protein, LIS3 Protein, TUBA3 Protein, Tuba-1 Protein, TUBA3D Protein, TUBA3E Protein, wu:fb37a10 Protein, 1t Protein, ALPHA 84C Protein, Alpha-Tubulin84B Protein, BEST:LD32507 Protein, CG1913 Protein, DM1alpha Protein, DTA1 Protein, Dmel\CG1913 Protein, T Protein, TBA1_DROME Protein, Talpha1 Protein, Tub Protein, Tub 84B Protein, Tub1C Protein, Tuba84B Protein, Tubalpha1 Protein, a-tub84B Protein, a1t Protein, aTub84B Protein, alpha-Tub Protein, alpha-Tub84B Protein, alpha-tub Protein, alpha-tub84B Protein, alpha-tubulin Protein, alpha-tubulin 84B Protein, alpha1 Protein, alpha1t Protein, alpha1tub Protein, alpha1tub84B Protein, alpha84B Protein, alphaT Protein, alphaTUB Protein, alphaTUB84B Protein, alphaTub Protein, alphaTub1 Protein, alphaTub84 Protein, alphat-1 Protein, alphatub Protein, alphatub84B Protein, anon-EST:Liang-1.59 Protein, anon-EST:Liang-2.30 Protein, chr3R:2914276..2914416 Protein, clone 1.59 Protein, clone 2.30 Protein, l(3)84Bd Protein, l(3)g3 Protein, ms(3)nc33 Protein, nc33 Protein, tub Protein, tubA84B Protein, tubalpha1 Protein, tubulin Protein, TUBA1A Protein, Tuba1a Protein, Tuba2 Protein, alphatub84 Protein, b-alpha-1 Protein, lis3 Protein, tuba1a Protein, tuba1a-a Protein, tuba1a-b Protein, tuba3 Protein, tubulin, alpha 1A Protein, tubulin alpha 1a Protein, tubulin, alpha 1a Protein, tubulin alpha-1B chain Protein, tubulin alpha-1A chain Protein, tubulin alpha-3 chain-like Protein, alpha-Tubulin at 84B Protein, tubulin alpha 1a L homeolog Protein, Tuba1a Protein, TUBA1A Protein, LOC608147 Protein, LOC100392817 Protein, LOC425049 Protein, tuba1a Protein, alphaTub84B Protein, LOC100051840 Protein, LOC100340483 Protein, LOC100726777 Protein, tuba1a.L Protein
    Background
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    Molecular Weight
    51.1 kDa Including tag.
    UniProt
    P68369
    Pathways
    Microtubule Dynamics, M Phase
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