STIM1 Protein (AA 235-685) (His tag)

Catalog No. ABIN3120956

Product Details

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Protein Type: Recombinant

Protein Characteristics: AA 235-685

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This STIM1 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: RYSKEHMKKM MKDLEGLHRA EQSLHDLQER LHKAQEEHRT VEVEKVHLEK KLRDEINLAK QEAQRLKELR EGTENERSRQ KYAEEELEQV REALRKAEKE LESHSSWYAP EALQKWLQLT HEVEVQYYNI KKQNAERQLL VAKEGAEKIK KKRNTLFGTF HVAHSSSLDD VDHKILTAKQ ALSEVTAALR ERLHRWQQIE ILCGFQIVNN PGIHSLVAAL NIDPSWMGST RPNPAHFIMT DDVDDMDEEI VSPLSMQSPS LQSSVRQRLT EPQLGLGSQR DLTHSDSESS LHMSDRQRVA PKPPQMGRAA DEALNAMPSN GSHRLIEGVH PGSLVEKLPD SPALAKKTFM ALNHGLDKAH SLMELNPSVP PGGSPLLDSS HSLSPSSPDP DTPSPVGDNR ALQGSRNTRI PHLAGKKAMA EEDNGSIGEE TDSSPGRKKF PLKIFKKPLK K
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Stim1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: Stim1 (STIM1 Products)

Synonyms: stim1 Protein, MGC76045 Protein, STIM1 Protein, si:dkey-24p1.5 Protein, MGC154748 Protein, si:ch211-112l17.2 Protein, Stim1 Protein, D11S4896E Protein, GOK Protein, TAM Protein, SIM Protein, stromal interaction molecule 1 Protein, stromal interaction molecule 1a Protein, stromal interaction molecule 1 L homeolog Protein, stromal interaction molecule 1b Protein, STIM1 Protein, stim1 Protein, stim1a Protein, stim1.L Protein, stim1b Protein, Stim1 Protein

Background: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1. Involved in enamel formation. Activated following interaction with TMEM110/STIMATE, leading to promote STIM1 conformational switch. {ECO:0000250|UniProtKB:Q13586}.

Molecular Weight: 51.8 kDa Including tag.

UniProt: P70302

Pathways: TCR Signaling, BCR Signaling