SAP30BP Protein (AA 1-308) (His tag)
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- Target See all SAP30BP Proteins
- SAP30BP (SAP30 Binding Protein (SAP30BP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-308
- Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SAP30BP protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MAGKKNVLSS LAIYAEYSDP ESDGETGVDA VGGATEEKGG LVSDAYGEDD FSRPGGDEDG YEEEEDENSK QSEDDDSETE KPEADDPKDN TEAEKRDPQE LVASFSERVR NMSPDEIKIP PEPPGRCSNH LQDKIQKLYE RKIKEGMDMN YIIQRKKEFR NPSIYEKLIQ FCAIDELGTN YPKDMFDPHG WSEDSYYEAL AKAQKIEMDK LEKAKKERTK IEFVTGTKKG TTTNATATST STASTAVADA QKRKSKWDSA IPVTTIAQPT ILTTTATLPA VVTVTTSASG SKTTVISAVG TIVKKAKQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Sap30bp Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SAP30BP Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SAP30BP (SAP30 Binding Protein (SAP30BP))
- Alternative Name
- Sap30bp (SAP30BP Products)
- Synonyms
- HCNGP Protein, HTRG Protein, HTRP Protein, 2700016D05Rik Protein, Hcngp Protein, RGD1565537 Protein, fb33d03 Protein, wu:fb33d03 Protein, zgc:55340 Protein, transcriptional regulator protein HCNGP Protein, Transcriptional regulator protein HCNGP Protein, SAP30 binding protein L homeolog Protein, SAP30 binding protein Protein, CpipJ_CPIJ000069 Protein, Bm1_27655 Protein, sap30bp.L Protein, SAP30BP Protein, Sap30bp Protein, sap30bp Protein
- Background
- Induces cell death. May act as a transcriptional corepressor of a gene related to cell survival (By similarity). May be involved in the regulation of beta-2-microglobulin genes. {ECO:0000250}.
- Molecular Weight
- 34.8 kDa Including tag.
- UniProt
- Q02614
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