METTL4 Protein (AA 1-471) (Strep Tag)

Catalog No. ABIN3121902

Product Details

Target: METTL4 (Methyltransferase Like 4 (METTL4))

Protein Type: Recombinant

Protein Characteristics: AA 1-471

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This METTL4 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSVVHHLPPG WLLDHLSFIN KVNYQLCQHQ ESFCSKNNPT SSVYMDSLQL DPGSPFGAPA MCFAPDFTTV SGNDDEGSCE VITEKYVFRS ELFNVTKPYI VPAVHKERQQ SNKNENLVTD YKQEVSVSVG KKRKRCIAFN QGELDAMEYH TKIRELILDG SSKLIQEGLR SGFLYPLVEK QDGSSGCITL PLDACNLSEL CEMAKHLPSL NEMELQTLQL MGDDVSVIEL DLSSQIIENN SSFSKMITLM GQKYLLPPQS SFLLSDISCM QPLLNCGKTF DAIVIDPPWE NKSVKRSNRY SSLSPQQIKR MPIPKLAAAD CLIVTWVTNR QKHLCFVKEE LYPSWSVEVV AEWYWVKITN SGEFVFPLDS PHKKPYECLV LGRVKEKTPL ALRNPDVRIP PVPDQKLIVS VPCVLHSHKP PLTEVLRDYI KPGGQCLELF ARNLQPGWMS WGNEVLKFQH MDYFIALESG C
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: METTL4 (Methyltransferase Like 4 (METTL4))

Alternative Name: Mettl4 (METTL4 Products)

Synonyms: HsT661 Protein, 2410198H06Rik Protein, A730091E08Rik Protein, AV296509 Protein, RGD1306451 Protein, methyltransferase like 4 Protein, METTL4 Protein, Mettl4 Protein

Background: N(6)-adenine-specific methyltransferase METTL4 (Methyltransferase-like protein 4) (N(6)-adenine-specific DNA methyltransferase METTL4) (EC 2.1.1.72) (snRNA (2'-O-methyladenosine-N(6)-)-methyltransferase METTL4) (EC 2.1.1.-),FUNCTION: N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA (PubMed:30982744). Acts as a N(6)-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation (By similarity). Internal m6A(m) methylation of snRNAs regulates RNA splicing (By similarity). Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine) (PubMed:30982744). The existence of N(6)-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)-adenine-specific DNA methyltransferase activity of METTL4 in vivo (By similarity). Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending (By similarity). N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (PubMed:30982744). {ECO:0000250|UniProtKB:Q8N3J2, ECO:0000269|PubMed:30982744}.

Molecular Weight: 53.3 kDa

UniProt: Q3U034