KLHL21 Protein (AA 1-597) (Strep Tag)

Catalog No. ABIN3121954

Product Details

Target: KLHL21 (Kelch-Like 21 (KLHL21))

Protein Type: Recombinant

Protein Characteristics: AA 1-597

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This KLHL21 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MERPAPLAVL PFSDPAHALS LLRGLSQLRA ERKFLDVTLE AAGGRDFPAH RAVLAAASPY FRAMFAGQLR ESRAERVRLH GVPPDMLQLL LDFSYTGRVA VSGDNAEPLL RAADLLQFPA VKEACGAFLQ QQLDLANCLD MQDFAEAFSC SGLASAAQRF ILRHVGELGA EQLERLPLAR LLRYLRDDGL CVPKEEAAYQ LALRWVRADP PRRAPHWPQL LEAVRLPFVR RFYLLAHVEA EPLVARCPPC LRLLREARDF QAARYDRHDR GPCPRMRPRP STGLAEILVL VGGCDQDCDE LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI VALGNDIYVT GGSDGSRLYD CVWRYNSSVN EWTEVAPMLK AREYHSSSVL DGLLYVVAAD STERYDHATD SWEALQPMTY PMDNCSTTAC RGRLYAIGSL AGKETMVIQC YDPDTDLWSL VNCGQLPPWS FAPKTVTLNG LMYFVRDDSA EVDVYNPTKD EWDKIPSMNQ VHVGGSLAVL GGKLYVSGGY DNTFELSDVV EAYDPETRAW SVVGRLPEPT FWHGSVSIFR QFMPQTPAGG RGFELNSGSN DVDAGYHRLP QNPEELH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KLHL21 (Kelch-Like 21 (KLHL21))

Alternative Name: Klhl21 (KLHL21 Products)

Synonyms: zgc:76933 Protein, 1810045K06Rik Protein, D330008A20 Protein, mKIAA0469 Protein, RGD1305863 Protein, kelch-like family member 21 Protein, kelch like family member 21 Protein, kelch like family member 21 L homeolog Protein, kelch-like 21 Protein, klhl21 Protein, KLHL21 Protein, klhl21.L Protein, Klhl21 Protein

Background: Kelch-like protein 21,FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome (By similarity). {ECO:0000250}.

Molecular Weight: 66.5 kDa

UniProt: Q3U410

Pathways: M Phase