CYP7B1 Protein (AA 1-507) (Strep Tag)

Catalog No. ABIN3122813

Product Details

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Protein Type: Recombinant

Protein Characteristics: AA 1-507

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CYP7B1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MQGATTLDAA SPGPLALLGL LFAATLLLSA LFLLTRRTRR PREPPLIKGW LPYLGMALKF FKDPLTFLKT LQRQHGDTFT VFLVGKYITF VLNPFQYQYV TKNPKQLSFQ KFSSRLSAKA FSVKKLLTDD DLNEDVHRAY LLLQGKPLDA LLETMIQEVK ELFESQLLKI TDWNTERIFA FCGSLVFEIT FATLYGKILA GNKKQIISEL RDDFFKFDDM FPYLVSDIPI QLLRNEESMQ KKIIKCLTSE KVAQMQGQSK IVQERQDLLK RYYRHDDPEI GAHHLGFLWA SLANTIPAMF WAMYYILRHP EAMEALRDEI DSFLQSTGQK KGPGISVHFT REQLDSLVCL ESTILEVLRL CSYSSIIREV QEDMNLSLES KSFSLRKGDF VALFPPLIHN DPEIFDAPKE FRFDRFIEDG KKKSTFFKGG KKLKTYVMPF GLGTSKCPGR YFAVNEMKLL LIMLLTYFDL EIIDRKPIGL NHSRMFLGIQ HPDSAVSFRY KAKSWRS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Alternative Name: Cyp7b1 (CYP7B1 Products)

Synonyms: CYP7B1 Protein, CBAS3 Protein, CP7B Protein, SPG5A Protein, AW261589 Protein, D3Ertd552e Protein, hct-1 Protein, cytochrome P450 family 7 subfamily B member 1 Protein, cytochrome P450, family 7, subfamily B, polypeptide 1 Protein, cytochrome P450, family 7, subfamily b, polypeptide 1 Protein, CYP7B1 Protein, cyp7b1 Protein, Cyp7b1 Protein

Background: Cytochrome P450 7B1 (24-hydroxycholesterol 7-alpha-hydroxylase) (EC 1.14.14.26) (25/26-hydroxycholesterol 7-alpha-hydroxylase) (EC 1.14.14.29) (3-hydroxysteroid 7-alpha hydroxylase) (Hippocampal transcript 1 protein) (HCT-1) (Oxysterol 7-alpha-hydroxylase),FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids (PubMed:9295351, PubMed:9144166, PubMed:10748047, PubMed:11067870). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR, NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase (PubMed:9295351, PubMed:9144166, PubMed:10748047, PubMed:11067870). Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids (PubMed:9295351, PubMed:9144166, PubMed:10748047, PubMed:11067870). Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response (PubMed:22999953). 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning (PubMed:9144166). Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives (By similarity). {ECO:0000250|UniProtKB:O75881, ECO:0000269|PubMed:10748047, ECO:0000269|PubMed:11067870, ECO:0000269|PubMed:22999953, ECO:0000269|PubMed:9144166, ECO:0000269|PubMed:9295351}.

Molecular Weight: 58.5 kDa

UniProt: Q60991

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Intracellular Steroid Hormone Receptor Signaling