ACVRL1 Protein (AA 141-502) (His tag)

Catalog No. ABIN3122921

Product Details

Target: ACVRL1 (Activin A Receptor Type II-Like 1 (ACVRL1))

Protein Type: Recombinant

Protein Characteristics: AA 141-502

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This ACVRL1 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: RVRRRQEKQR DLHSDLGESS LILKASEQAD SMLGDFLDSD CTTGSGSGLP FLVQRTVARQ VALVECVGKG RYGEVWRGSW HGESVAVKIF SSRDEQSWFR ETEIYNTVLL RHDNILGFIA SDMTSRNSST QLWLITHYHE HGSLYDFLQR QTLEPQLALR LAVSAACGLA HLHVEIFGTQ GKPAIAHRDL KSRNVLVKSN LQCCIADLGL AVMHSQSSDY LDIGNNPRVG TKRYMAPEVL DEHIRTDCFE SYKWTDIWAF GLVLWEIARR TIINGIVEDY RPPFYDMVPN DPSFEDMKKV VCVDQQTPTI PNRLAADPVL SGLAQMMREC WYPNPSARLT ALRIKKTLQK LSHNPEKPKV IH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Acvrl1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ACVRL1 (Activin A Receptor Type II-Like 1 (ACVRL1))

Alternative Name: Acvrl1 (ACVRL1 Products)

Synonyms: ACVRL1 Protein, vgb Protein, alk1 Protein, zehn1109 Protein, hm:zehn1109 Protein, ACVRLK1 Protein, ALK-1 Protein, ALK1 Protein, HHT Protein, HHT2 Protein, ORW2 Protein, SKR3 Protein, TSR-I Protein, R-3 Protein, R3 Protein, SETHKIR Protein, AI115505 Protein, AI427544 Protein, Acvrlk1 Protein, Alk1 Protein, activin A receptor like type 1 Protein, activin A receptor type II-like 1 Protein, activin A receptor, type II-like 1 Protein, ACVRL1 Protein, acvrl1 Protein, Acvrl1 Protein

Background: Type I receptor for TGF-beta family ligands BMP9/GDF2 and BMP10 and important regulator of normal blood vessel development. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. May bind activin as well. {ECO:0000250|UniProtKB:P37023}.

Molecular Weight: 42.1 kDa Including tag.

UniProt: Q61288