GFRAL Protein (AA 20-349) (His tag)

Catalog No. ABIN3123818

Product Details

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Protein Type: Recombinant

Protein Characteristics: AA 20-349

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This GFRAL protein is labelled with His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MQTNDCAHLI QKCLIDANGC EQSWRSMEDT CLTPGDSCKI NNSLHCNLSI QALVEKNFQF KECLCMDDLH CTVNKLFGKK CTNKTDNMEK DNKDKWNLTT TPFYHGFKQM QSCLEVTEAC VGDVVCNAQL ALYLKACSAN GNLCDVKHCQ AAIRFFYQNM PFNTAQMLAF CDCAQSDIP CQQSKETLHS KPCALNIVPP PTCLSVIHTC RNDELCRTHY RTFQTECWPH ITGKCHEDET CISMLGKQDL TCSGSESCRA AFLGTFGTVL QVPCACRGVT QAEEHVCMIF QHMLHSKSC FNYPTPNVKD ISSYEKKNSK EITLTGFNSF FNGHHHHHH
Sequence with tag.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Gfral Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

C-terminal His-tag

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20 mM Hepes, pH 7.4, 100 mM NaCl

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Alternative Name: Gfral (GFRAL Products)

Synonyms: RGD1565220 Protein, C6orf144 Protein, GRAL Protein, UNQ9356 Protein, bA360D14.1 Protein, AY457637 Protein, Gral Protein, GDNF family receptor alpha like Protein, Gfral Protein, GFRAL Protein

Molecular Weight: 38.0 kDa Including tag.

UniProt: Q6SJE0