ANKZF1 Protein (AA 1-748) (Strep Tag)

Catalog No. ABIN3124291

Product Details

Target: ANKZF1 (Ankyrin Repeat and Zinc Finger Domain Containing 1 (ANKZF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-748

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ANKZF1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAKPAGDAAV GSRSGELFLP SVSSSATSPA PSAAPAPASV SLLSLNGEAP LIRGLSLVSQ APGEALAWAP RTSCPGENTS SGGKVSPYSL EISEKLFCSA CDQIFQNHQE QREHYKLDWH RFNLKQRLKN KPLLSASDFE QQSSTGDLSS ISGSDDTDSS SEEDLLPLDE GRAESEKPNR PPGFYPHRVL FKNAQGQFLY AYRCVLGPHQ IPPEKAELLL QNLQNGGPRY YVVLMAAAGH FAGAIFQGRE VVAHKTFHRY TVRAKRGTAQ GLQDAHGRAS RSAGANLRRY NEAMLYKDVR NLLAGPIWSK ALGEAETVLF RAPRSGRSLF FGGQGAPLQR DDPRLWDIPL TTRRPTFGEL QRVLHKLTTL QVYDEDPREM VRFHSPETHW KPVREERKKD TEKEKTKVPS DANKPLGQDE EPLKQGSESQ EEDGSEVELE LVELTLGTLD LREFEVLPKR RRRRKKKERS QEQQCGAHGP LPQQPQDEPF SQPTQEVETP LDTLVYEAKA PGQPELWDTL LAACRAGEVE VLKLQLATGL VDPGVKSLLN APLGSGGFTL LHAAAAAGRG LVVRLLLEAG ADPTVQDSRA RPPYTVAADK STRNEFRRFM EKNLDAYDYN KARVPGPLTQ EMEARQATRK KEQKAARRQR EQQQRKQREQ EEQEQEEQRR FAALSDREKR ALAAERRLAA QLGAPSPPVP DSAVASAGRC WSCGVSLQGL IPFHYLDFSF CSTRCLRDHR SQAGRPSS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ANKZF1 (Ankyrin Repeat and Zinc Finger Domain Containing 1 (ANKZF1))

Alternative Name: Ankzf1 (ANKZF1 Products)

Synonyms: ANKZF1 Protein, ZNF744 Protein, RGD1359242 Protein, 1300008P06Rik Protein, 2810025E10Rik Protein, D1Ertd161e Protein, im:7148662 Protein, ankyrin repeat and zinc finger domain containing 1 Protein, ANKZF1 Protein, ankzf1 Protein, Ankzf1 Protein

Background: TRNA endonuclease ANKZF1 (EC 3.1.-.-) (Ankyrin repeat and zinc finger domain-containing protein 1),FUNCTION: Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation. The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts. ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs. ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1. Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress. {ECO:0000250|UniProtKB:Q9H8Y5}.

Molecular Weight: 83.0 kDa

UniProt: Q80UU1