Metadherin (MTDH) (AA 1-579) protein (rho-1D4 tag)

Details for Product No. ABIN3124367
Protein Name
  • MGC75963
  • 2610103J23Rik
  • 3D3
  • 3D3/Lyric
  • AEG-1
  • AV353288
  • D8Bwg1112e
  • Lyric
  • AEG1
  • LYRIC
  • LYRIC/3D3
  • metadherin
  • mtdh
  • MTDH
  • Mtdh
Protein Characteristics
AA 1-579
2
1
1
Origin
Mouse (Murine)
6
1
Source
Insect Cells
3
1
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
rho-1D4 tag
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Sequence MAARSWQDEL AQQAEEGSAR LRELLSVGLG FLRTELGLDL GLEPKRYPGW VILVGTGALG LLLLFLLGYG WAAACAGARK KRRSPPRKRE EAAPPTPAPD DLAQLKNLRS EEQKKKNRKK LPEKPKPNGR TVEVPEDEVV RNPRSITAKQ APETDKKNEK SKKNKKKSKS DAKAVQNSSR HDGKEVDEGA WETKISHREK RQQRKRDKVL TDSGSLDSTI PGIENIITVT TEQLTTASFP VGSKKNKGDS HLNVQVSNFK SGKGDSTLQV SSRLNENLTV NGGGWSEKSV KLSSQLSEEK WNSVPPASAG KRKTEPSAWT QDTGDTNANG KDWGRNWSDR SIFSGIGSTA EPVSQSTTSD YQWDVSRNQP YIDDEWSGLN GLSSADPSSD WNAPAEEWGN WVDEDRASLL KSQEPISNDQ KVSDDDKEKG EGALPTGKSK KKKKKKKKQG EDNSHTQDTE DLEKDTREEL PVNTSKARPK QEKACSLKTM STSDPAEVLI KNSQPVKTLP PAISAEPSIT LSKGDSDNSS SQVPPMLQDT DKPKSNAKQN SVPPSQTKSE TNWESPKQIK KKKKARRET
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Mtdh Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Protein Name
Background Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance (By similarity). {ECO:0000250}.
Molecular Weight 65.0 kDa Including tag.
UniProt Q80WJ7
Pathways Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Metadherin (MTDH) (AA 1-579) protein (rho-1D4 tag) (ABIN3124367) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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