BRF1 Protein (AA 1-676) (Strep Tag)

Catalog No. ABIN3125967

Product Details

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-676

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This BRF1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MTGRVCRGCG GTDIELDAAR GDAVCTGCGS VLEDNIIVSE VQFVENSGGG SSAVGQFVSL DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGSQL QLNQHCLDTA FNFFKMAVSK HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GALANLSKDG SGEDATSSPR CEEDTEDEEL EAAASHMNKD FYRELLGDDD GSEAAGDPDG GSRPLALESL LGPLPTAASL GISDSIRECI SSPSGDPKDS SGDGELDLSG IDDLEIDRYI LNESEARVKA ELWMRENAEY LREQKEKEAR IAKEKELGIY KEHKPKKSCK RREPILASTA GEAIEKMLEQ KKISSKINYS VLRDLNSKGG GSPPRDDSQP PERASTKKLS RRKRATTRNS ADPGTSTGKR LRPLLSAQPA KKAAVGEALL LSSPALGAEP VKPSAVLVES GPVPYHPEED ADEEDAEDED GEPCVSALQM MGGNDYGCDG DEDDGY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Alternative Name: Brf1 (BRF1 Products)

Synonyms: BRF Protein, BRF-1 Protein, GTF3B Protein, TAF3B2 Protein, TAF3C Protein, TAFIII90 Protein, TF3B90 Protein, TFIIIB90 Protein, hBRF Protein, DDBDRAFT_0189159 Protein, DDBDRAFT_0216283 Protein, DDB_0189159 Protein, DDB_0216283 Protein, 2510002F24Rik Protein, mTFIIIB90 Protein, BRF1 Protein, ZFP36L1 Protein, CG31256 Protein, CG4155 Protein, CG5419 Protein, Dmel\\CG31256 Protein, TFIIIB Protein, brf Protein, brf1 Protein, wu:fi47f03 Protein, zgc:55543 Protein, fi40c10 Protein, wu:fi40c10 Protein, zgc:63497 Protein, BRF1, RNA polymerase III transcription initiation factor subunit Protein, transcription initiation factor IIB Protein, general transcription factor IIB Protein, cilia and flagella associated protein 20 Protein, transcription factor IIb, putative Protein, transcription factor IIB Protein, transcription factor IIb Protein, Transcription factor iib, putative Protein, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit Protein, CG31256 gene product from transcript CG31256-RA Protein, BRF1, RNA polymerase III transcription initiation factor a Protein, BRF1, RNA polymerase III transcription initiation factor b Protein, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit L homeolog Protein, BRF1 Protein, gtf2b Protein, GTF2B Protein, cfap20 Protein, PFE0415w Protein, Tb09.160.4220 Protein, PVX_097980 Protein, CpipJ_CPIJ011738 Protein, CpipJ_CPIJ014799 Protein, CpipJ_CPIJ018710 Protein, PKH_102450 Protein, Brf1 Protein, Brf Protein, brf1a Protein, brf1b Protein, brf1.L Protein

Background: Transcription factor IIIB 90 kDa subunit (TFIIIB90) (mTFIIIB90) (B-related factor 1) (BRF-1),FUNCTION: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. {ECO:0000250}.

Molecular Weight: 73.8 kDa

UniProt: Q8CFK2