ASB2 Protein (AA 1-634) (Strep Tag)

Catalog No. ABIN3126220

Product Details

Target: ASB2 (Ankyrin Repeat and SOCS Box-Containing 2 (ASB2))

Protein Type: Recombinant

Protein Characteristics: AA 1-634

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ASB2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MATEISTRGR QRAIGHEEYS LYSSLSEEEL LQMAIEQSLA DKTRGPTPAE ASASSQTNHQ PGHFHPWTRS PSSPENPPAR APLGLFQGVM QKYSSNLFKT SQMAAMDPVL KAIKEGDEEA LKIMIQDGKN LAEPNKEGWL PLHEAAYYGQ LGCLKVLQQA YPGTIDQRTL QEETALYLAT CREHLDCLLS LLQAGAEPDI SNKSRETPLY KACERKNAEA VRILVRYNAD ANHRCNRGWT ALHESVSRND LEVMEILVSG GAKVEAKNVY SITPLFVAAQ SGQLEALRFL AKHGADINTQ ASDSASALYE ASKNEHEDVV EFLLSQGADA NKANKDGLLP LHVASKKGNY RIVQMLLPVT SRTRVRRSGI SPLHLAAERN HDAVLEALLA ARFDVNAPLA PERARLYEDR RSSALYFAVV NNNVYATELL LLAGADPNRD VISPLLVAIR HGCLRTMQLL LDHGANIDAY IATHPTAFPA TIMFAMKCLS LLKFLMDLGC DGEPCFSCLY GNGPHPPAPR PGRFHDAPVD DKAPSVVQFC EFLSAPEVSR WAGPIIDVLL DYVGNVQLCS RLKEHIDSFE DWAVIKEKAE PPRPLAHLCR LRVRKAIGKY RIKLLDTLPL PGRLIRYLKY ENTQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ASB2 (Ankyrin Repeat and SOCS Box-Containing 2 (ASB2))

Alternative Name: Asb2 (ASB2 Products)

Synonyms: ASB-2 Protein, 1110008E15Rik Protein, ASB2 Protein, asb2 Protein, zgc:77223 Protein, ankyrin repeat and SOCS box containing 2 Protein, ankyrin repeat and SOCS box-containing 2 Protein, ankyrin repeat and SOCS box containing 2b Protein, ASB2 Protein, Asb2 Protein, asb2b Protein

Background: Ankyrin repeat and SOCS box protein 2 (ASB-2),FUNCTION: Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Mediates Notch-induced ubiquitination and degradation of substrates including TCF3/E2A and JAK2 (By similarity). Required during embryonic heart development for complete heart looping (PubMed:32179481). Required for cardiomyocyte differentiation (By similarity). {ECO:0000250|UniProtKB:Q96Q27, ECO:0000269|PubMed:32179481}., FUNCTION: [Isoform 1]: Involved in myogenic differentiation and targets filamin FLNB for proteasomal degradation but not filamin FLNA (PubMed:26343497). Also targets DES for proteasomal degradation (PubMed:26343497). Acts as a negative regulator of skeletal muscle mass (PubMed:27182554). {ECO:0000269|PubMed:26343497, ECO:0000269|PubMed:27182554}., FUNCTION: [Isoform 2]: Targets filamins FLNA and FLNB for proteasomal degradation (PubMed:23632887). This leads to enhanced adhesion of hematopoietic cells to fibronectin (By similarity). Required for FLNA degradation in immature cardiomyocytes which is necessary for actin cytoskeleton remodeling, leading to proper organization of myofibrils and function of mature cardiomyocytes (PubMed:29374072). Required for degradation of FLNA and FLNB in immature dendritic cells (DC) which enhances immature DC migration by promoting DC podosome formation and DC-mediated degradation of the extracellular matrix (PubMed:23632887). Does not promote proteasomal degradation of tyrosine-protein kinases JAK1 or JAK2 in hematopoietic cells (By similarity). {ECO:0000250|UniProtKB:Q96Q27, ECO:0000269|PubMed:23632887, ECO:0000269|PubMed:29374072}.

Molecular Weight: 70.2 kDa

UniProt: Q8K0L0