BRD3 Protein (AA 1-726) (Strep Tag)

Catalog No. ABIN3126330

Product Details

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Protein Type: Recombinant

Protein Characteristics: AA 1-726

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This BRD3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSTTAAAPTG IPAVPGPVNP PPPEVSNPSK PGRKTNQLQY MQNVVVKTLW KHQFAWPFYQ PVDAIKLNLP DYHKIIKNPM DMGTIKKRLE NNYYWSASEC MQDFNTMFTN CYIYNKPTDD IVLMAQALEK IFLQKVAQMP QEEVELLPPA PKGKGRKPAA GAQNAGSQQV AAVSSVSPAT PFQNIPPTVS QTPVIAATPV PTITANVTSV PVPPPAAPPP PATPIVPVVP PTPPVVKKKG VKRKADTTTP TTSAITASRS ESPPPLSEPK QAKVVARRES GGRPIKPPKK DLEDGEVPQH AGKKGKLSEH LRHCDSILRE MLSKKHAAYA WPFYKPVDAE ALELHDYHDI IKHPMDLSTV KRKMDSREYP DAQGFAADIR LMFSNCYKYN PPDHEVVAMA RKLQDVFEMR FAKMPDEPME APALPAPTAP IVSKGAESSR SSEESSSDSG SSDSEEERAT RLAELQEQLK AVHEQLAALS QAPVNKPKKK KEKKEKEKKK KDKDKDKEKE KHKAKSEEEK KAKAAPAAKQ AQQKKAPTKK ANSTTTASRQ LKKGGKQASA SYDSEEEEEG LPMSYDEKRQ LSLDINRLPG EKLGRVVHII QSREPSLRDS NPDEIEIDFE TLKPTTLREL ERYVKSCLQK KQRKPLSTSG KKQAAKSKEE LAQEKKKELE KRLQDVSGQL NSKKPTKKEK SGSAPSGGPS RLSSSSSSES ASSSSSGSSS DSSDSE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Alternative Name: Brd3 (BRD3 Products)

Synonyms: ORFX Protein, RING3L Protein, 2410084F24Rik Protein, AW060456 Protein, Fsrg2 Protein, RINGL3 Protein, brd3 Protein, fi36b10 Protein, wu:fa13h06 Protein, wu:fc01d09 Protein, wu:fc74a08 Protein, wu:fi04h10 Protein, wu:fi36b10 Protein, BRD3 Protein, ring3l Protein, fa28f05 Protein, wu:fa28f05 Protein, wu:fc56a11 Protein, wu:fj66d12 Protein, zgc:77289 Protein, bromodomain containing 3 Protein, bromodomain containing 3a Protein, bromodomain containing 3 S homeolog Protein, bromodomain-containing protein 3 Protein, bromodomain containing 3b Protein, BRD3 Protein, Brd3 Protein, brd3a Protein, brd3.S Protein, LOC100410652 Protein, LOC100354641 Protein, brd3b Protein

Background: Bromodomain-containing protein 3 (Bromodomain-containing FSH-like protein FSRG2),FUNCTION: Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:21536911). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (By similarity). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:21536911). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (PubMed:21536911, PubMed:21555453). {ECO:0000250|UniProtKB:Q15059, ECO:0000269|PubMed:21536911, ECO:0000269|PubMed:21555453, ECO:0000269|PubMed:32895492}.

Molecular Weight: 79.8 kDa

UniProt: Q8K2F0

Pathways: Chromatin Binding