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PPHLN1 Protein (AA 1-381) (His tag)

Crystallography grade PPHLN1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3126333
  • Target See all PPHLN1 Proteins
    PPHLN1 (Periphilin 1 (PPHLN1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-381
    Origin
    • 5
    • 1
    Mouse
    Source
    • 2
    • 2
    • 2
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This PPHLN1 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MWSEGRYDYD RLPRERVPPR SHPSDGYHRV VNVVPKRPPL LDKRPPLLDK RPPLLARPDE GGYSRYYSHV DCRVCDEGRS FSHDRRSGPS HSGDESGYRW LRDDHSTSRQ PDYRDMRDGF RRKSFYSSHY SRDRSPHKRD APFFRESPVG RKDSPHSRSG SSVSSRSYSP ERSRTHSFHQ SQHRKSSRVG ASYKRQNEAI RGRGKERSIQ SVKTSRDASP SSSSAVASSK ALDKPSRLTE KELAEAESKW ANETLEKSDE SNLAEMNEFE AGSTAPLFID QTEEPESNTV DGTELYEDSQ LSNRSKAIAS KTKEIEQVYR QDCETFGMVV KMLIEKDPSL EKSVQFALRQ NLHEIGERCV EELKRFITEY DNSARDFGDP F
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Pphln1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PPHLN1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PPHLN1 (Periphilin 1 (PPHLN1))
    Alternative Name
    Pphln1 (PPHLN1 Products)
    Synonyms
    MGC75908 Protein, MGC81987 Protein, wu:fb83c09 Protein, zgc:158246 Protein, si:ch211-155a11.2 Protein, HSPC232 Protein, 1110063K05Rik Protein, 1600022A19Rik Protein, CR Protein, HSPC206 Protein, periphilin 1 Protein, periphilin 1 S homeolog Protein, PPHLN1 Protein, pphln1 Protein, pphln1.S Protein, Pphln1 Protein
    Background
    Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression. The HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. In the HUSH complex, contributes to the maintenance of the complex at chromatin. Acts as a transcriptional corepressor and regulates the cell cycle, probably via the HUSH complex. May be involved in epithelial differentiation by contributing to epidermal integrity and barrier formation. {ECO:0000250|UniProtKB:Q8NEY8}.
    Molecular Weight
    44.8 kDa Including tag.
    UniProt
    Q8K2H1
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