MANBA Protein (AA 20-879) (His tag)

Catalog No. ABIN3126340

Product Details

Target: MANBA (Mannosidase, beta A, Lysosomal (MANBA))

Protein Type: Recombinant

Protein Characteristics: AA 20-879

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This MANBA protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: GPSYSLSGSW RVSNGNGSLE LPATVPGYVH SALHQHGLIQ DPYYRFNDLN YRWISLDNWT YSTEFKIPFN LSEWQKVKLI FDGVDTVAEI LFNNVTIGKT DNMFTGYSFD ITNVVKDVNS LKLQFRSAVQ YAECQSKAHT SYRVPPECPP VEQKGECHVN FIRKAQCSFS WDWGPSFPSQ GIWKDVRIEA YNIAHLDYLT FLPVYDNASQ AWNIEIKASF DVASSKSVGG QVTVAIPQLK TQQTNDIELQ QEQRIVKLLV KIRKDVAVET WWPRGHGNQT GYNMTILFAL DGGLKIEKAA KVYFRTVQLI EEGIKGSPGL SFYFKINGLP IFLKGSNWIP ADSFQDKVTS DRLQLLFQSV VDANMNTLRV WGGGIYEQDE FYALCDELGI MVWQDFMFAS ALYPTEPGFL ASVRKEVTYQ VRRLKSHPSI IIWSGNNENE VALSVNWFHV NPRDMKTYID DYVTLYVKNI RKIVLSEDKS RPFIASSPTN GMKTMEEGWI SYDPYSIQYG DIHFYNYADD CWNWKIFPKA RLVSEYGYQS WPSFSTLEKV SSQEDWAYNS RFSLHRQHHE DGNHQMLHQV KMHFKLPQGT DPLRTFKDTI YLTQVMQAQC IKTETEFYLR SRSEIVDGKG HTMGALYWQL NDIWQAPSWA SLEYGGKWKM LHYFARRFFA PLLPVGFEDE GVFYVYGVSD LHKDHHTQLT VRLHHWSSPK PLCSLVNSSI VVKAGEAVVL FQMPVSELLK RCRGCTRETC VVSFYFSTDK ELFSPTNYHF LSSLKDAKGL LEANITVNIS QKGNVFVFDL ETSAVAPFVW LDVGSIPGRF SDNGFLMIRK KLSVLFYPWK PTSKSELQQA FSVTSLTDTY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Manba Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MANBA (Mannosidase, beta A, Lysosomal (MANBA))

Alternative Name: Manba (MANBA Products)

Synonyms: zgc:55493 Protein, zgc:76896 Protein, MGC82825 Protein, SC2H4.14 Protein, MANB1 Protein, 2410030O07Rik Protein, B930014J03Rik Protein, Bmn Protein, mannosidase, beta A, lysosomal Protein, mannosidase beta Protein, mannosidase, beta A, lysosomal L homeolog Protein, Probable beta-mannosidase Protein, beta-mannosidase Protein, Beta-mannosidase Protein, manba Protein, MANBA Protein, manba.L Protein, C33G3.4 Protein, SCO6232 Protein, bmnA Protein, THAPSDRAFT_262343 Protein, Jden_1756 Protein, PAAG_08337 Protein, Ndas_4827 Protein, Spirs_1196 Protein, VDBG_07992 Protein, RBRH_03524 Protein, Manba Protein

Background: Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides. {ECO:0000250|UniProtKB:O00462}.

Molecular Weight: 99.7 kDa Including tag.

UniProt: Q8K2I4