Interferon Regulatory Factor 2 Binding Protein-Like (IRF2BPL) (AA 1-775) protein (Strep Tag)

Catalog No. ABIN3126416

Product Details

Target: Interferon Regulatory Factor 2 Binding Protein-Like (IRF2BPL)

Protein Type: Recombinant

Protein Characteristics: AA 1-775

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: Strep Tag

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSAAQVSSSR RQSCYLCDLP RMPWAMIWDF SEPVCRGCVN YEGADRIEFV IETARQLKRA HGCFQDGRSP GPPPPVGVKT VALSAKEAAA AAAAAQQQQQ QQQQQQQQLN HVDGSTKPAV LAAPSGLERY GLSAAAAAAA AAAAVEQRSR FEYPPPPVSL GSSSHAARLP NGLGGPNGFP KPAPEEGPPE LNRQSPNSSS AATSVASRRG THSGLVTGLP NPGGGGGPQL TVPPNLLPQT LLNGPASAAV LPPPHGLGGS RGPPTPAPPG APGGPACLGG PPGVSATVSS APSSTSSTVA EVGVGAAGKR PGSVSSTDQE RELKEKQRNA EALAELSESL RNRAEEWANK PKMVRDTLLT LAGCTPYEVR FKKDHSLLGR VFAFDAVSKP GMDYELKLFI EYPTGSGNVY SSASGVAKQM YQDCMKDFGR GLSSGFKYLE YEKKHGSGDW RLLGDLLPEA VRFFKEGVPG ADMLPQPYLD ASCPMLPTAL VSLSRAPSAP PGTGALPPAA PTGRGAASSL RKRKASPEPP DSAESALKLG EEQQRQQWMA NQSEALKLTM SAGGFAAPGH SAGGPPPPPP PLGPHSNRTT PPESAPQNGP SPMAALMSVA DTLGTAHSPK DGSSVHSTTA SARRNSSSPV SPASVPGQRR LASRNGDLNL QVAPPPPSAH PGMDQVHPQN IPDSPMANSG PLCCTICHER LEDTHFVQCP SVPSHKFCFP CSRESIKAQG ATGEVYCPSG EKCPLVGSNV PWAFMQGEIA TILAGDVKVK KERDP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Interferon Regulatory Factor 2 Binding Protein-Like (IRF2BPL)

Alternative Name: Irf2bpl (IRF2BPL Products)

Synonyms: C14orf4 Protein, EAP1 Protein, 6430527G18Rik Protein, Eap1 Protein, Pqcp Protein, RGD1310994 Protein, C14orf4L Protein, interferon regulatory factor 2 binding protein like Protein, interferon regulatory factor 2 binding protein-like Protein, IRF2BPL Protein, Irf2bpl Protein

Background: Probable E3 ubiquitin-protein ligase IRF2BPL (EC 2.3.2.27) (Enhanced at puberty protein 1) (Interferon regulatory factor 2-binding protein-like),FUNCTION: Probable E3 ubiquitin protein ligase involved in the proteasome-mediated ubiquitin-dependent degradation of target proteins. Through the degradation of CTNNB1, functions downstream of FOXF2 to negatively regulate the Wnt signaling pathway. Probably plays a role in the development of the central nervous system and in neuronal maintenance (By similarity). Also acts as a transcriptional regulator of genes controlling female reproductive function. May play a role in gene transcription by transactivating GNRH1 promoter and repressing PENK promoter (By similarity). {ECO:0000250|UniProtKB:Q5EIC4, ECO:0000250|UniProtKB:Q9H1B7}.

Molecular Weight: 80.6 kDa

UniProt: Q8K3X4