PANK1 Protein (AA 1-548) (Strep Tag)

Catalog No. ABIN3126456

Product Details

Target: PANK1 (Pantothenate Kinase 1 (PANK1))

Protein Type: Recombinant

Protein Characteristics: AA 1-548

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PANK1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MRGRRAPRPG STEVPAAARD ADTLRARAAS PVRGAQLAED VGTPTGGGEE RRGHQLPTAA PRLRESKPQG GSEDRGTADR DLQRGCQSRS PRTAPPVPGM GDRGAQHERA ALQSPGAPEG AAAAVNGLLH NGFHPSAASS RDPPAPRFQL PSELQPQPLF AQHDSPAKKC RLRRRMDSGR KNRPPFPWFG MDIGGTLVKL VYFEPKDITA EEEQEEVENL KSIRKYLTSN TAYGKTGIRD VHLELKNLTM CGRKGNLHFI RFPTCAMHLF IQMGSEKNFS SLHTTLCATG GGAFKFEEDF RMIADLQLHK LDELDCLIQG LLYVDSVGFN GKPECYYFEN PTNPELCQKK PYCLDNPYPM LLVNMGSGVS ILAVYSKDNY KRVTGTSLGG GTFLGLCCLL TGCETFEEAL DMAAKGDSTN VDKLVKDIYG GDYERFGLQG SAVASSFGNM MSKEKRESIS KEDLARATLV TITNNIGSIA RMCALNENID RVVFVGNFLR INMVSMKLLA YAMDFWSKGQ LKALFLEHEG YFGAVGALLE LFKMTDTQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PANK1 (Pantothenate Kinase 1 (PANK1))

Alternative Name: Pank1 (PANK1 Products)

Synonyms: PANK Protein, 4632412I06Rik Protein, 5430426F23Rik Protein, Pank Protein, Pank1a Protein, Pank1b Protein, panK1beta Protein, PANK1 Protein, im:7149302 Protein, wu:fb51a11 Protein, zgc:158746 Protein, ATCOAA Protein, ATPANK1 Protein, T13D8.31 Protein, T13D8_31 Protein, pantothenate kinase 1 Protein, DKFZp469J1021 Protein, fb79e08 Protein, zgc:63556 Protein, wu:fb79e08 Protein, pantothenate kinase 1 Protein, pantothenate kinase 1b Protein, pantothenate kinase 1a Protein, PANK1 Protein, Pank1 Protein, pank1b Protein, EHI_183060 Protein, pank1a Protein

Background: Pantothenate kinase 1 (mPank) (mPank1) (EC 2.7.1.33) (Pantothenic acid kinase 1),FUNCTION: Catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis (PubMed:20559429, PubMed:22815849). Required for the hepatic CoA increase during the switch from glucose to fatty acid oxidation that occurs in the fasting state (PubMed:20559429). {ECO:0000269|PubMed:20559429, ECO:0000269|PubMed:22815849}., FUNCTION: [Isoform 1]: Catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis. {ECO:0000269|PubMed:12095677, ECO:0000269|PubMed:17379144}., FUNCTION: [Isoform 2]: Catalyzes the phosphorylation of pantothenate to generate 4'-phosphopantothenate in the first and rate-determining step of coenzyme A (CoA) synthesis. {ECO:0000269|PubMed:10625688, ECO:0000269|PubMed:12095677, ECO:0000269|PubMed:16040613, ECO:0000269|PubMed:17379144}.

Molecular Weight: 60.1 kDa

UniProt: Q8K4K6

Pathways: Ribonucleoside Biosynthetic Process