GTF2H3 Protein (AA 1-309) (Strep Tag)

Catalog No. ABIN3126990

Product Details

Target: GTF2H3 (General Transcription Factor IIH, Polypeptide 3, 34kD (GTF2H3))

Protein Type: Recombinant

Protein Characteristics: AA 1-309

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This GTF2H3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAADEDELNL LVIIVDTNPI WWGKQALKES QFTLSKCMDA VMVLANSHLF MNRSNQLAVI ASHIQESRLL YPGKNGGLGD FFGDPGNALP DCNPSGSKDG KYELLTVANE VIAEEIKDLM TKSDIKGQHT ETLLAGSLAK ALCYIHRVNK AVKDNQEMKS RILVIKAAED SALQYMNFMN VIFAAQKQNI LIDACVLDSD SGLLQQACDI TGGLYLKVPQ MPSLLQYLLW VFLPDQDQRS QLILPPPIHV DYRAACFCHR SLIEIGYVCS VCLSIFCNFS PICTTCETAF KISLPPVLKA KKKKQKVSL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GTF2H3 (General Transcription Factor IIH, Polypeptide 3, 34kD (GTF2H3))

Alternative Name: Gtf2h3 (GTF2H3 Products)

Synonyms: DDBDRAFT_0167192 Protein, DDBDRAFT_0230196 Protein, DDB_0167192 Protein, DDB_0230196 Protein, btf2 Protein, tfb4 Protein, tfiih Protein, 34kDa Protein, 5033417D07Rik Protein, BTF2 Protein, BTF2 p34 Protein, C730029A10 Protein, D5Ertd679e Protein, TFIIH Protein, GB12027 Protein, fc26a07 Protein, zgc:92759 Protein, wu:fc26a07 Protein, P34 Protein, TFB4 Protein, general transcription factor IIH, polypeptide 3 Protein, general transcription factor IIH subunit 3 Protein, gtf2h3 Protein, GTF2H3 Protein, Gtf2h3 Protein, LOC411231 Protein

Background: General transcription factor IIH subunit 3 (Basic transcription factor 2 34 kDa subunit) (BTF2 p34) (General transcription factor IIH polypeptide 3) (TFIIH basal transcription factor complex p34 subunit),FUNCTION: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. {ECO:0000250|UniProtKB:Q13889}.

Molecular Weight: 34.2 kDa

UniProt: Q8VD76

Pathways: DNA Damage Repair