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CBS Protein (AA 2-561) (His tag)

Crystallography grade CBS Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Catalog No. ABIN3127404
  • Target See all CBS Proteins
    CBS (Cystathionine-beta-Synthase (CBS))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-561
    Origin
    • 6
    • 2
    • 2
    • 1
    Mouse
    Source
    • 4
    • 3
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CBS protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequence
    PSGTSQCEDG SAGGFQHLDM HSEKRQLEKG PSGDKDRVWI RPDTPSRCTW QLGRAMADSP HYHTVLTKSP KILPDILRKI GNTPMVRINK ISKNAGLKCE LLAKCEFFNA GGSVKDRISL RMIEDAERAG NLKPGDTIIE PTSGNTGIGL ALAAAVKGYR CIIVMPEKMS MEKVDVLRAL GAEIVRTPTN ARFDSPESHV GVAWRLKNEI PNSHILDQYR NASNPLAHYD DTAEEILQQC DGKLDMLVAS AGTGGTITGI ARKLKEKCPG CKIIGVDPEG SILAEPEELN QTEQTAYEVE GIGYDFIPTV LDRAVVDKWF KSNDEDSFAF ARMLIAQEGL LCGGSSGSAM AVAVKAAREL QEGQRCVVIL PDSVRNYMSK FLSDKWMLQK GFMKEELSVK RPWWWRLRVQ ELSLSAPLTV LPTVTCEDTI AILREKGFDQ APVVNESGAI LGMVTLGNML SSLLAGKVRP SDEVCKVLYK QFKPIHLTDT LGTLSHILEM DHFALVVHEQ IQSRDQAWSG VVGGPTDCSN GMSSKQQMVF GVVTAIDLLN FVAAREQTQT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cbs Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product CBS Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CBS (Cystathionine-beta-Synthase (CBS))
    Alternative Name
    Cbs (CBS Products)
    Synonyms
    hip4 Protein, GB12529 Protein, CBS Protein, DDBDRAFT_0189727 Protein, DDBDRAFT_0191292 Protein, DDB_0189727 Protein, DDB_0191292 Protein, cbs Protein, AI047524 Protein, AI303044 Protein, HIP4 Protein, cb442 Protein, wu:fb37g05 Protein, wu:fm61c07 Protein, wu:fq06c06 Protein, zgc:113704 Protein, cystathionine-beta-synthase L homeolog Protein, cystathionine-beta-synthase Protein, cystathionine beta-synthase Protein, cystathionine beta-synthase CBS Protein, cystathionine beta synthase Protein, cystathionine-beta-synthase S homeolog Protein, cystathionine-beta-synthase b Protein, cbs.L Protein, cbs Protein, Cbs Protein, CBS Protein, CNA06170 Protein, Tb11.02.5400 Protein, cysB Protein, LOC100635325 Protein, cbs.S Protein, cbsb Protein
    Background
    Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine (By similarity). Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons (By similarity). {ECO:0000250|UniProtKB:P32232, ECO:0000250|UniProtKB:P35520}.
    Molecular Weight
    62.4 kDa Including tag.
    UniProt
    Q91WT9
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