FOXI1 Protein (AA 1-372) (His tag)
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- Target See all FOXI1 Proteins
- FOXI1 (Forkhead Box I1 (FOXI1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-372
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This FOXI1 protein is labelled with His tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
- Sequence
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MSSFDLPAPS PPRCSPQFPS IGQEPPEMNL YYENFFHPQG MPSPQRPTSF EGGGEYGTTP NPYLWLNGPA MTPPPYLPGT NASPFLPQAY GMQRQLLPSD LGWLPIPSQE ELMKLVRPPY SYSALIAMAI HGAPDQRLTL SQIYQYVADN FPFYNKSKAG WQNSIRHNLS LNDCFKKVPR DEDDPGKGNY WTLDPNCEKM FDNGNFRRKR KRKSDSSSST SSLASEKTEN GLLASSPKPT EPQEVLDTAS PDTTTSSPEK RSSPAPSGTP CLNNFLSTMT AYVSGTNPIS RSVATPGLSS EPIDKMGQNS LNFNSYTPLT NLSSHGNGGE WANPVATNAL GYGGSVFNQF SPHFYNSINT NGILFPREGT EV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Foxi1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product FOXI1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- FOXI1 (Forkhead Box I1 (FOXI1))
- Alternative Name
- Foxi1 (FOXI1 Products)
- Synonyms
- FKH10 Protein, FKHL10 Protein, FREAC-6 Protein, FREAC6 Protein, HFH-3 Protein, HFH3 Protein, 5830401E05Rik Protein, Fkh10 Protein, Hfh3 Protein, cb724 Protein, foo Protein, Xfoxi1a Protein, ema Protein, foxi1a Protein, foxi1e Protein, xema Protein, xfoxi1 Protein, FoxI1e Protein, Xema Protein, fkhl10 Protein, freac6 Protein, hfh3 Protein, FOXI1 Protein, forkhead box I1 Protein, forkhead box i1 Protein, forkhead box I1 L homeolog Protein, forkhead box protein I1 Protein, FOXI1 Protein, Foxi1 Protein, foxi1 Protein, foxi1.L Protein, CC1G_14832 Protein
- Background
- Transcriptional activator required for the development of normal hearing, sense of balance and kidney function. Required for the expression of SLC26A4/PDS, JAG1 and COCH in a subset of epithelial cells and the development of the endolymphatic system in the inner ear. Also required for the expression of SLC4A1/AE1, SLC4A9/AE4, ATP6V1B1 and the differentiation of intercalated cells in the epithelium of distal renal tubules. {ECO:0000269|PubMed:12642503, ECO:0000269|PubMed:15173882}.
- Molecular Weight
- 41.8 kDa Including tag.
- UniProt
- Q922I5
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