RIOK1 Protein (AA 1-567) (Strep Tag)

Catalog No. ABIN3127717

Product Details

Target: RIOK1 (RIO Kinase 1 (RIOK1))

Protein Type: Recombinant

Protein Characteristics: AA 1-567

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This RIOK1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MDCSLDRMAS VVPGQFDDAD SSDSENKELQ PIHAEDGGVL LKSLQNAAAE VKGDAETDEE DDYDDDDDWY LDDATGKLTK GCTWNGGSNY QANRQTSNYN SAKMSTPIDK SLRKFENKIN LNKLNVTDSV TNKVTVKLRQ KEAESYRIKD KADRATVEQV LDPRTRMILF KLLHKDHISE IHGCISTGKE ANVYYASTPS GESRAIKIYK TSILMFKDRD KYVTGEFRFR RGYCKGNPRK MVRTWAEKEM RNLCRLKTAN IPCPEPIRLR SHVLLMGFIG KDDMPAPLLK NVQLSESKAR ELYLQVIQYM RKMYQDARLV HADLSEFNML YHGGDVYIID VSQSVEHDHP HALEFLRKDC TNVNDFFSKH AVAVMTVREL FDFVTDPSIT ADNMDAYLEK AMEIASQRTK EEKTSQDHVD EEVFKQAYIP RTLNEVKNYE RDVDIMMRLK EEDMALNTQQ DNILYQTVMG LKKDLSGVQK VPALLESEVK EETCFGSDDA GGSECSDTVS EEQEDQAGCR NHIADPDIDK KERKKMVKEA QREKRKNKIP KHVKKRKEKT AKAKKGK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RIOK1 (RIO Kinase 1 (RIOK1))

Alternative Name: Riok1 (RIOK1 Products)

Synonyms: AD034 Protein, RRP10 Protein, bA288G3.1 Protein, 3110046C13Rik Protein, 5430416A05Rik Protein, Ad034 Protein, RIO kinase 1 Protein, RIO kinase 1 (yeast) Protein, Riok1 Protein, RIOK1 Protein

Background: Serine/threonine-protein kinase RIO1 (EC 2.7.11.1) (EC 3.6.3.-) (RIO kinase 1),FUNCTION: Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (By similarity). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (By similarity). {ECO:0000250|UniProtKB:G0S3J5, ECO:0000250|UniProtKB:Q12196, ECO:0000250|UniProtKB:Q9BRS2}.

Molecular Weight: 64.9 kDa

UniProt: Q922Q2