AASDHPPT Protein (AA 1-309) (Strep Tag)

Catalog No. ABIN3128303

Product Details

Target: AASDHPPT (Aminoadipate-Semialdehyde Dehydrogenase-phosphopantetheinyl Transferase (AASDHPPT))

Protein Type: Recombinant

Protein Characteristics: AA 1-309

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This AASDHPPT protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MVFPAKRLCV VPSMEGVRWA FSCGTWLPSR AEWLLAMRSI QPEEKERIGK FVFARDAKAA LAGRLMIRKL VAEKLNIPWD HIRLQRTSKG KPVLAKDSLN PYPNFNFNIS HQGDYAVLAA EPEVQVGIDI MKTSFPGRGS IPEFFHIMKR KFTKKEWETI RSFNDEWTQL DMFYRHWALK ESFIKAIGVG LGFEMQRLEF DVSPLNMDIG QVYKETCLIL DGEEEKEWAF EESKIDEHHF VAVAVRKPDG SRHQNVSYQD DSKLSQRKFT ILNFNDLVAS AIPMTPEDPS FWDCFCFTEE ILIRNGTKS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: AASDHPPT (Aminoadipate-Semialdehyde Dehydrogenase-phosphopantetheinyl Transferase (AASDHPPT))

Alternative Name: Aasdhppt (AASDHPPT Products)

Synonyms: AASD-PPT Protein, LYS2 Protein, LYS5 Protein, 2010309J24Rik Protein, 2810407B07Rik Protein, CGI-80 Protein, AASDHPPT Protein, DDBDRAFT_0186752 Protein, DDBDRAFT_0304576 Protein, DDB_0186752 Protein, DDB_0304576 Protein, aasdhppt Protein, aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase Protein, aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase S homeolog Protein, AASDHPPT Protein, Aasdhppt Protein, Bm1_31065 Protein, DDB_G0285927 Protein, aasdhppt Protein, aasdhppt.S Protein

Background: L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (EC 2.7.8.7) (4'-phosphopantetheinyl transferase) (Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase) (AASD-PPT),FUNCTION: Catalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine moiety from coenzyme A, regardless of whether the CoA is presented in the free thiol form or as an acetyl thioester, to a serine residue of a broad range of acceptors including the acyl carrier domain of FASN. {ECO:0000250|UniProtKB:Q9NRN7}.

Molecular Weight: 35.8 kDa

UniProt: Q9CQF6