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FAM119A Protein (AA 1-218) (His tag)

Crystallography grade FAM119A Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3128331
  • Target See all FAM119A Proteins
    FAM119A (Family With Sequence Similarity 119A (FAM119A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-218
    Origin
    • 5
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 4
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This FAM119A protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    MALVPYEESA AIGLQKFHKP LATFSFANHT IQIRQDWRQL GVAAVVWDAA VVLSMYLEMG AVELRGCSAV ELGAGTGLVG IVAALLGAQV TITDRKVALE FLKSNVEANL PPHIQPKAVV KELTWGQNLE SFSPGEFDLI LGADVIYLED TFTDLLQTLG HLCSNNSVIL LACRIRYERD SNFLTMLERQ FTVSKVHYDP EKDVHIYKAQ KRNQREDL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mettl21A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product FAM119A Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    FAM119A (Family With Sequence Similarity 119A (FAM119A))
    Alternative Name
    Mettl21A (FAM119A Products)
    Synonyms
    2310038H17Rik Protein, AI464204 Protein, Fam119a Protein, FAM119A Protein, HCA557b Protein, zgc:110528 Protein, RGD1311824 Protein, methyltransferase like 21A Protein, Mettl21a Protein, METTL21A Protein, mettl21a Protein
    Background
    Protein-lysine methyltransferase that selectively trimethylates residues in heat shock protein 70 (HSP70) family members. Contributes to the in vivo trimethylation of Lys residues in HSPA1 and HSPA8. In vitro methylates 'Lys-561' in HSPA1, 'Lys-564' in HSPA2, 'Lys-585' in HSPA5, 'Lys-563' in HSPA6 and 'Lys-561' in HSPA8 (By similarity). {ECO:0000250}.
    Molecular Weight
    25.2 kDa Including tag.
    UniProt
    Q9CQL0
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