CHTOP Protein (AA 2-249) (His tag)

Catalog No. ABIN3128644

Product Details

Target: CHTOP (Chromatin Target of PRMT1 (CHTOP))

Protein Type: Recombinant

Protein Characteristics: AA 2-249

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CHTOP protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: AAQSAPKVVL KSTTKMSLNE RFTNMLKNKQ PMPVNIRASM QQQQQLASAR NRRLAQQMEN RPSVQAALKL KQKSLKQRLG KSNIQARLGR PIGALARGAI GGRGLPIIQR GLPRGGLRGG RATRTLLRGG MSLRGQNLLR GGRAVAPRMG LRRGGVRGRG GPGRGGLGRG AMGRGGIGGR GRGMIGRGRG GFGGRGRGRG RGRGALTRPV LTKEQLDNQL DAYMSKTKGH LDAELDAYMA QTDPETND
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Chtop Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CHTOP (Chromatin Target of PRMT1 (CHTOP))

Alternative Name: Chtop (CHTOP Products)

Synonyms: C1orf77 Protein, FL-SRAG Protein, FOP Protein, RP1-178F15.2 Protein, SRAG Protein, SRAG-3 Protein, SRAG-5 Protein, pp7704 Protein, 2500003M10Rik Protein, C81330 Protein, Fop Protein, Srag Protein, C25H1orf77 Protein, C3H1orf77 Protein, c1orf77 Protein, chtop Protein, fop Protein, srag Protein, fb17a05 Protein, wu:fb17a05 Protein, wu:fi05e09 Protein, zgc:55292 Protein, zgc:153909 Protein, chromatin target of PRMT1 Protein, chromatin target of PRMT1 S homeolog Protein, chromatin target of PRMT1 protein Protein, chromatin target of PRMT1a Protein, chromatin target of PRMT1b Protein, CHTOP Protein, Chtop Protein, chtop.S Protein, LOC100344922 Protein, chtopa Protein, chtopb Protein

Background: Plays an important role in the ligand-dependent activation of estrogen receptor target genes (By similarity). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859). Required for the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC methylates H4R3 and transactivates genes involved in glioblastomagenesis (PubMed:25284789). {ECO:0000250|UniProtKB:Q9Y3Y2, ECO:0000269|PubMed:20688955, ECO:0000269|PubMed:22872859, ECO:0000269|PubMed:25284789}.

Molecular Weight: 27.4 kDa Including tag.

UniProt: Q9CY57