SERBP1 Protein (AA 2-407) (His tag)

Catalog No. ABIN3128645

Product Details

Target: SERBP1 (SERPINE1 mRNA Binding Protein 1 (SERBP1))

Protein Type: Recombinant

Protein Characteristics: AA 2-407

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SERBP1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: PGHLQEGFGC VVTNRFDQLF DDESDPFEVL KAAENKKKEA GGGGVGGPGA KSAAQAAAQT NSNAAGKQLR KESQKDRKNP LPPSVGVADK KEETQPPVAL KKEGIRRVGR RPDQQLQGDG KLIDRRAERR PPRERRFEKP LEEKGEGGEF SVDRPIIERP IRGRGGLGRG RGGRGRGMGR GDGFDSRGKR EFDRHSGSDR SSFSHYSGLK HEDKRGGSGS HNWGTVKDEL TESPKYIQKQ ISYNCSDLDQ SNVTEETPEG EEHPVADTEN KENEVEEVKE EGPKEMTLDE WKAIQNKDRA KVEFNIRKPN EGADGQWKKG FVLHKSKSEE AHAEDSVMDH HFRKPANDIT SQLEINFGDL GRPGRGGRGG RGGRGRGGRP NRGSRTDKSS ASAPDVDDPE AFPALA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Serbp1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SERBP1 (SERPINE1 mRNA Binding Protein 1 (SERBP1))

Alternative Name: Serbp1 (SERBP1 Products)

Synonyms: CHD3IP Protein, HABP4L Protein, PAI-RBP1 Protein, PAIRBP1 Protein, 1200009K13Rik Protein, 9330147J08Rik Protein, AL022786 Protein, Pairbp1 Protein, Pai-Rbp1 Protein, Rda288 Protein, cgi-55 Protein, chd3ip Protein, habp4l Protein, pai-rbp1 Protein, pairbp1 Protein, rda288 Protein, hm:zeh0245 Protein, serbp1 Protein, wu:fa12d05 Protein, wu:fb36e07 Protein, wu:fc16f12 Protein, wu:fj13e12 Protein, zgc:55489 Protein, SERPINE1 mRNA binding protein 1 Protein, serpine1 mRNA binding protein 1 Protein, Serpine1 mRNA binding protein 1 Protein, SERPINE1 mRNA binding protein 1 L homeolog Protein, SERPINE1 mRNA binding protein 1a Protein, SERBP1 Protein, Serbp1 Protein, serbp1.L Protein, serbp1a Protein

Background: May play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay (By similarity). {ECO:0000250}.

Molecular Weight: 45.5 kDa Including tag.

UniProt: Q9CY58