CHAF1B Protein (AA 1-572) (Strep Tag)

Catalog No. ABIN3128814

Product Details

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Protein Type: Recombinant

Protein Characteristics: AA 1-572

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CHAF1B protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MKVITCEIAW HNKEPVYSLD FQHGATWKIH RLASAGVDTA VRIWKLERGP DGKAIVEFLS NLARHTKAVN VVRFSPTGEI LASGGDDAVI LLWKMNDSKE PEQIAFQDEE EAQLNKENWT VVKTLRGHLE DVYDICWATD GNLMTSASVD NTVIIWDVSK GQKISIFNEH KSYVQGVTWD PLGQYIATLS CDRVLRIYNT QKKRVAFNIS KMLSGQGPEG EARSFRMFHD DSMKSFFRRL SFTPDGSLLL TPAGCMESGE NVTNTTYVFS RKHLKRPIAH LPCPGKATLA VRCCPVYFEL RPVAETEKAS EEPSPELVNL PYRMVFAVAS EDSVLLYDTQ QSFPFGYVSN IHYHTLSDIS WSSDGAFLAI SSTDGYCTFV TFEKGELGIP LKEKPVLSIR TPDTAKKAKN QTHQGSSPGS RSVEGTPSNR TQDPSSPCTT PSPTTQSPAP SAIKDSPSAI PAGKSPLPQP SEEKTLQPAG QNMKAPQPRR VTLNTLQTWG KTAPRRINLT PLKTDTVPNP QPNSGTAPST EEVQPEAPGE PPEEPPELKR PRLEEREGDA QNLAPDDSSK TV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Alternative Name: Chaf1b (CHAF1B Products)

Synonyms: CHAF1B Protein, wu:fd07c09 Protein, wu:fe36g06 Protein, wu:fv38g09 Protein, zgc:56096 Protein, CAF-1 Protein, CAF-IP60 Protein, CAF1 Protein, CAF1A Protein, CAF1P60 Protein, MPHOSPH7 Protein, MPP7 Protein, 2600017H24Rik Protein, C76145 Protein, CAF-I p60 Protein, CAF-Ip60 Protein, caf-1 Protein, CAF-1P60 Protein, chromatin assembly factor 1 subunit B Protein, chromatin assembly factor 1, subunit B Protein, chromatin assembly factor 1, subunit B (p60) Protein, chromatin assembly factor 1 subunit B L homeolog Protein, CHAF1B Protein, chaf1b Protein, Chaf1b Protein, chaf1b.L Protein

Background: Chromatin assembly factor 1 subunit B (CAF-1 subunit B) (Chromatin assembly factor I p60 subunit) (CAF-I 60 kDa subunit) (CAF-I p60),FUNCTION: Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA, histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer (By similarity). {ECO:0000250|UniProtKB:Q13112, ECO:0000250|UniProtKB:Q5R1S9}.

Molecular Weight: 63.1 kDa

UniProt: Q9D0N7