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PHPT1 Protein (AA 1-124) (His tag)

Crystallography grade PHPT1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Catalog No. ABIN3129476
  • Target See all PHPT1 Proteins
    PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-124
    Origin
    • 9
    • 2
    • 1
    • 1
    • 1
    Mouse
    Source
    • 8
    • 4
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This PHPT1 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequence
    MAADLGQIPD VDIDSDGVFK YVLIRVHLAE PSGDPAKECK EIVRGYKWAE YHADIYDKVS GELQRNGYDC ECLGGGRISH QSQDRKIHVY GYSMGYGRAQ HSVSTEKIKA KYPDYEVTWA DDGY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Phpt1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PHPT1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
    Alternative Name
    Phpt1 (PHPT1 Products)
    Synonyms
    wu:fb50e08 Protein, zgc:101086 Protein, MGC131298 Protein, PHP14 Protein, CGI-202 Protein, HSPC141 Protein, RP11-216L13.10 Protein, bA216L13.10 Protein, 1700008C22Rik Protein, Php14 Protein, phosphohistidine phosphatase 1 Protein, phosphohistidine phosphatase 1 L homeolog Protein, 14 kDa phosphohistidine phosphatase-like Protein, LOC424401 Protein, phpt1 Protein, PHPT1 Protein, phpt1.L Protein, LOC100511240 Protein, Phpt1 Protein
    Background
    Exhibits phosphohistidine phosphatase activity. {ECO:0000250}.
    Molecular Weight
    15.0 kDa Including tag.
    UniProt
    Q9DAK9
    Pathways
    Positive Regulation of Peptide Hormone Secretion
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