APBB2 Protein (AA 1-760) (Strep Tag)

Catalog No. ABIN3129584

Product Details

Target: APBB2 (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 2 (APBB2))

Protein Type: Recombinant

Protein Characteristics: AA 1-760

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This APBB2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSEVLPADSG VGTLAVFMAS SGSTDIANRN SPATPPNTLN LRSSHNELLN AEIKHSDAKN STPPKCRKKY ALTNIQAAMG LSDPAVQPLL GNGSANIKLV KNGENQLRKA AEQGQQDPNK NLSPAAVINL TSEKLEVKDP HPQESSGCEI LPSQPRRTKS FLNYYADLET SARELGQNLG PCQGVGEEKA QPGPGQAPVV IGNGDLLPQK PNKPQSSPED GQVATVSSSP ETKKDHPKTG AKTDCALHRI QNLAPSDEES SWTTLSQDSA SPSSPDETDI WSDHSFQTDP DLPPGWKRVN DIAGTYYWHI PTGTTQWERP VSIPADLHGS RKGSLSSVTP SPTPENEKQP WSDFAVLNGG KINSDIWKDL HAATVNPDPS LKEFEGATLR YASLKLRNAP HGDDDDSCSI NSDPEAKCFA VRSLGWVEMA EEDLAPGKSS VAVNNCIRQL SYCKNDIRDT VGIWGEGKDM YLSLENDMLS LVDPMDRSVL HSQPIVNIRV WGVGRDNGRE RDFAYVARDK DTRILKCHVF RCDTPAKAIA TSLHEICSKI MAERKNAKAL ACSSLQERTN MSLDVPLQVD FPTPKTELVQ KFRVQYLGML PVDRPVGMDT LNSAIENLMT SSSKEDWPSV NMNVADATVT VISEKNEEEV LVECRVRFLS FMGVGKDVHT FAFIMDTGNQ RFECHVFWCE PNAANVSEAV QAACMLRYQK CLVARPPSQK VRPPPPPADS VTRRVTTNVK RGVLSLIDTL KQKRPVTETP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: APBB2 (Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 2 (APBB2))

Alternative Name: Apbb2 (APBB2 Products)

Synonyms: FE65L Protein, FE65L1 Protein, 2310007D03Rik Protein, Rirl1 Protein, TR2L Protein, Zfra Protein, RGD1562438 Protein, APBB2 Protein, fe65l Protein, fe65l1 Protein, DKFZp469I236 Protein, si:ch211-201g8.2 Protein, amyloid beta precursor protein binding family B member 2 Protein, amyloid beta (A4) precursor protein-binding, family B, member 2 Protein, amyloid beta (A4) precursor protein-binding, family B, member 2b Protein, APBB2 Protein, Apbb2 Protein, apbb2 Protein, apbb2b Protein

Background: Amyloid beta precursor protein binding family B member 2 (Amyloid-beta (A4) precursor protein-binding family B member 2) (Protein Fe65-like 1),FUNCTION: Plays a role in the maintenance of lens transparency, and may also play a role in muscle cell strength (PubMed:25757569, PubMed:27734846). Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning (PubMed:27734846). Activates transcription of APP (By similarity). {ECO:0000250|UniProtKB:Q92870, ECO:0000269|PubMed:25757569, ECO:0000269|PubMed:27734846}.

Molecular Weight: 83.2 kDa

UniProt: Q9DBR4