PSMG2 Protein (AA 1-264) (Strep Tag)

Catalog No. ABIN3129944

Product Details

Target: PSMG2 (Proteasome (Prosome, Macropain) Assembly Chaperone 2 (PSMG2))

Protein Type: Recombinant

Protein Characteristics: AA 1-264

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PSMG2 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequence: MFVPCGESVP DLTNFTLLMP AVSVGNVGQL AIDLIISTLN MCKIGYFYTD CLVPMVGNNP YATEEENSNE LSINTEVYSL PSKKLVVLQL RSIFIKYKSK SFCEKLLAWV ESSGCARIIV LSSSHSYHRN DAQLRSTPFR YLLTPCLQKS VQNKIKSLNW LEMEKSRCIP EMSDSEFCIR IPGGGITKTL YDESCSKEIQ MAVLLKFVSE GDNIPDAVSL VEYLNEWLQI IKPCNDGPMA SALPWKIPSS WRLLFGSGLP PALF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PSMG2 (Proteasome (Prosome, Macropain) Assembly Chaperone 2 (PSMG2))

Alternative Name: Psmg2 (PSMG2 Products)

Synonyms: clast3 Protein, TNFSF5IP1 Protein, tnfsf5ip1 Protein, DDBDRAFT_0167646 Protein, DDBDRAFT_0304396 Protein, DDB_0167646 Protein, DDB_0304396 Protein, CLAST3 Protein, HCCA3 Protein, HsT1707 Protein, MDS003 Protein, PAC2 Protein, 1700017I17Rik Protein, AW545363 Protein, Clast3 Protein, Tnfsf5ip1 Protein, wu:fb51b11 Protein, zgc:73278 Protein, proteasome (prosome, macropain) assembly chaperone 2 L homeolog Protein, proteasome assembly chaperone 2 Protein, proteasome (prosome, macropain) assembly chaperone 2 Protein, clast3-like protein Protein, psmg2.L Protein, PSMG2 Protein, psmg2 Protein, AT3G18940 Protein, psmG2 Protein, Tsp_07987 Protein, Psmg2 Protein

Background: Proteasome assembly chaperone 2 (CD40 ligand-activated specific transcript 3) (Tumor necrosis factor superfamily member 5-induced protein 1),FUNCTION: Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization (By similarity). {ECO:0000250|UniProtKB:Q969U7}.

Molecular Weight: 29.5 kDa

UniProt: Q9EST4

Pathways: Monocarboxylic Acid Catabolic Process