SLC9A3R2 Protein (AA 1-337) (Strep Tag)

Catalog No. ABIN3130001

Product Details

Target: SLC9A3R2 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 Regulator 2 (SLC9A3R2))

Protein Type: Recombinant

Protein Characteristics: AA 1-337

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC9A3R2 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MAAPESLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTQLLVVDK ETDEELCRRQ LTCTEEMAHR GLPPAHNPWE PKPDWACSGS LGSDTGQKDV NGPPRELRPR LCHLRRGPQG YGFNLHSDKS RPGQYIRSVD PGSPASHSGL RAQDRLIEVN GQNVEGLRHA EVVARIKAQE DEARLLVVDP ETDEHFKRLR VVPTEEHVEG PLPSPVTNGT SPAQLNGGSV CSSRSDLPGS EKDNEDGSTW KRDPFQESGL HLSPTAAEAK EKARATRVNK RAPQMDWNRK REIFSNF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC9A3R2 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 Regulator 2 (SLC9A3R2))

Alternative Name: Nherf2 (SLC9A3R2 Products)

Synonyms: E3KARP Protein, NHE3RF2 Protein, NHERF-2 Protein, NHERF2 Protein, OCTS2 Protein, SIP-1 Protein, SIP1 Protein, TKA-1 Protein, 0610011L07Rik Protein, 1200011K07Rik Protein, 2010007A20Rik Protein, E3karp Protein, Octs2 Protein, Sip-1 Protein, Sip1 Protein, Sryip1 Protein, Tka-1 Protein, nherf-2 Protein, nherf2 Protein, slc9a3r2 Protein, SLC9A3 regulator 2 Protein, solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2 Protein, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2 Protein, SLC9A3 regulator 2 S homeolog Protein, SLC9A3R2 Protein, Slc9a3r2 Protein, slc9a3r2.S Protein

Background: Na(+)/H(+) exchange regulatory cofactor NHE-RF2 (NHERF-2) (NHE3 kinase A regulatory protein E3KARP) (Octs2) (SRY-interacting protein 1) (SIP-1) (Sodium-hydrogen exchanger regulatory factor 2) (Solute carrier family 9 isoform A3 regulatory factor 2) (Tyrosine kinase activator protein 1) (TKA-1),FUNCTION: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus (By similarity). {ECO:0000250}.

Molecular Weight: 37.4 kDa

UniProt: Q9JHL1