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Kv2.2 Protein (AA 425-907) (His tag)

Crystallography grade KCNB2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3130918
$16,803.00
Plus shipping costs $45.00 and $20.00 dry ice
Shipping to: United States
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  • Target See all Kv2.2 (KCNB2) Proteins
    Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 425-907
    Origin
    • 3
    • 2
    Mouse
    Source
    • 5
    Insect Cells
    Purification tag / Conjugate
    This Kv2.2 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    KEQKRQEKAI KRREALERAK RNGSIVSMNL KDAFARSMEL IDVAVEKAGE SANTKDSVDD NHLSPSRWKW ARKALSETSS NKSYENKYQE VSQNDSHEHL NNTSSSSPQH LSAQKLEMLY NEITKTQPHS HPNPDCQEQP ERPCVYEEEI EMEEVICPQE QLAVAQTEVI VDMKSTSSID SFTSCATDFT ETERSPLPPP SASHLQMKFP TDLPGTDEHQ RARAPPFLTL SRDKGPAARE AAVDYAPIDI TVNLDAGASH GPLQPDSASD SPKSSLKGSN PLKSRSLKVN FQENRASAPQ TPPSTARPLP VTTADFPLTT PQHMSTILLE EALPQGQPPL LEADDSAHCQ GPSKGFSPRF PKQKLFPFSS RERRSFTEID TGEDEDFLDL QRSRPDKQAD PSPNCLADKP GDARDSLREE GCVGSSSPQN TDHNCRQDIY QAVGEVKKDS SQEGYKMENH LFAPEIHSNP GDTGHCPTRE TSM
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Kcnb2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Kv2.2 (KCNB2) (Potassium Voltage-Gated Channel, Shab-Related Subfamily, Member 2 (KCNB2))
    Alternative Name
    Kcnb2 (KCNB2 Products)
    Synonyms
    KCNB2 Protein, si:ch211-193n21.1 Protein, Kv2.2 Protein, 9630047L19Rik Protein, BB130875 Protein, XShab12 Protein, kcnb2 Protein, KV2.2 Protein, potassium voltage-gated channel subfamily B member 2 Protein, potassium voltage-gated channel, Shab-related subfamily, member 2 Protein, potassium channel, voltage gated Shab related subfamily B, member 2 Protein, potassium voltage gated channel, Shab-related subfamily, member 2 Protein, potassium channel, voltage gated Shab related subfamily B, member 2 S homeolog Protein, KCNB2 Protein, kcnb2 Protein, LOC100051372 Protein, Kcnb2 Protein, kcnb2.S Protein
    Background
    Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain and smooth muscle cells. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient. Homotetrameric channels mediate a delayed-rectifier voltage-dependent outward potassium current that display rapid activation and slow inactivation in response to membrane depolarization. Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNB1, channel properties depend on the type of alpha subunits that are part of the channel. Can also form functional heterotetrameric channels with other alpha subunits that are non-conducting when expressed alone, such as KCNS1 and KCNS2, creating a functionally diverse range of channel complexes. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Contributes to the delayed-rectifier voltage-gated potassium current in cortical pyramidal neurons and smooth muscle cells. {ECO:0000250|UniProtKB:Q63099}.
    Molecular Weight
    54.5 kDa Including tag.
    UniProt
    A6H8H5
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